|Product Overview:||Recombinant Human CD70(Gln39-Pro193) fused with HA tag at N-terminal was expressed in E. coli.|
|Description:||CD27 ligand (CD27L), also named CD70, is a type II transmembrane glycoprotein belonging to the TNF superfamily (TNFSF) and has been designated TNFSF7. Human CD27L cDNA encodes a 193 amino acid (aa) residue protein with a 17 aa N-terminal cytoplasmic domain, a 21 aa transmembrane domain and a 155 aa C-terminal extracellular domain. Human and mouse CD27L share approximated 56% aa sequence identity. CD27L is expected to exist as non-covalent homotrimers. CD70 is constitutively expressed on medullary thymic epithelial cells (mTECs) in human and mouse and on certain unconventional APCs in the mouse intestine. CD70 is also exclusively expressed on DCs, B cells, conventional- and regulatory T cells and NK cells but only after their activation. CD70 expression is highly regulated by antigen through Pattern Recognition Receptors (PRRs), T cell and B cell antigen receptors, and further tuned by cytokines such as IL-1 alpha,
IL-12, TNF alpha, prostaglandin E2 and by CD28 and CD40 co-stimulation. Ligation of CD27 on T cells provides co-stimulatory signals and promotes T cell proliferation, antigen-specific CD4+ and CD8+ T cells clonal expansion, effector & memory T-cell differentiation, and effector T-cell survival & T-cell memory formation. Ligation of CD27 on mouse B cell and NK cells promotes the B- and NK-cell response by supporting B-cell expansion in the germinal center and increasing IFN-gamma levels in NK cells, in turn enhancing NK cytotoxic capability, which can contribute to tumor control. Overall, CD27/CD70 co-stimulatory system promote T, B and NK cell responses and particularly the CTL response, and is a target in cancer immunotherapy.
|Predicted N Terminal:||Tyr|
|Form:||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Bio-activity:||50 for this effect is 5-25 ng/mL in the presence of Mouse Anti-Hemagglutinin/HA Peptide Monoclonal Antibody.|
|Molecular Mass:||Predicted Molecular Mass: 23 kDa
SDS-PAGE: 24-32 kDa, reducing conditions
|Endotoxin:||<0.1 EU per 1 μg of the protein by the LAL method.|
|Purity:||>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.|
|Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
|Reconstitution:||Reconstitute at 200 μg/mL in PBS.|
|Gene Name:||CD70 CD70 molecule [ Homo sapiens ]|
|Synonyms:||CD70; CD70 molecule; CD27LG, TNFSF7, tumor necrosis factor (ligand) superfamily, member 7; CD70 antigen; CD27L; CD27-L; CD27 ligand; Ki-24 antigen; surface antigen CD70; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7; CD27LG; TNFSF7;|
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