Recombinant Human CD80 protein, Avi & His-tagged, Biotinylated

• Cat.No.: CD80-1280H
• Product Overview: Biotinylated Human B7-1 (recommended for biopanning), His Tag is expressed from human 293 cells (HEK293). It contains AA Val 35 - Asn 242 (Accession # P33681-1). Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.

Specification

• Description: B7-1 and B7-2, together with their receptors CD28 and CTLA­4, constitute one of the dominant co-stimulatory pathways that regulate T­ and B­cell responses. Although both CTLA­4 and CD28 can bind to the same ligands, CTLA­4 binds to B7­1 and B7­2 with a 20 ­ 100 fold higher affinity than CD28 and is involved in the down­regulation of the immune response. B-lymphocyte activation antigen B7-1 (referred to as B7) also known as cluster of Differentiation 80 (CD80), is a member of cell surface immunoglobulin superfamily and is expressed on activated B cells, activated T cells, macrophages and dendritic cells. It is the ligand for two different proteins on the T cell surface: CD28 (for autoregulation and intercellular association) and CTLA-4 (for attenuation of regulation and cellular disassociation). CD80 works in tandem with CD86 to prime T cells. CD80 plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages. CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
• Source: HEK293
• Species: Human
• Tag: Avi & His
• Predicted N Terminal: Val 35
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 26.5 kDa. The protein migrates as 44-54 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: Val 35 - Asn 242
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20°C or lower.
  Please avoid repeated freeze-thaw cycles.
  No activity loss is observed after storage at:
  4-8°C for 12 months in lyophilized state;
  -70°C for 3 months under sterile conditions after reconstitution
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD80 CD80 molecule [ Homo sapiens (human) ]
• Official Symbol: CD80
• Synonyms: B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1; B-lymphocyte activation antigen B7; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CTLA-4 counter-receptor B7.1; activation B7-1 antigen; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; T-lymphocyte activation antigen CD80
• Gene ID: 941
• mRNA Refseq: NM_005191.3
• Protein Refseq: NP_005182.1
• UniProt ID: P33681