Recombinant Human CDC25A Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CDC25A-5283H
• Product Overview: CDC25A MS Standard C13 and N15-labeled recombinant protein (NP_001780) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 59.1 kDa
• AA Sequence: MELGPEPPHRRRLLFACSPPPASQP VVKALFGASAAGGLSPVTNLTVTMD QLQGLGSDYEQPLEVKNNSNLQRMG SSESTDSGFCLDSPGPLDSKENLEN PMRRIHSLPQKLLGCSPALKRSHSD SLDHDIFQLIDPDENKENEAFEFKK PVRPVSRGCLHSHGLQEGKDLFTQR QNSAPARMLSSNERDSSEPGNFIPL FTPQSPVTATLSDEDDGFVDLLDGE NLKNEEETPSCMASLWTAPLVMRTT NLDNRCKLFDSPSLCSSSTRSVLKR PERSQEESPPGSTKRRKSMSGASPK ESTNPEKAHETLHQSLSLASSPKGT IENILDNDPRDLIGDFSKGYLFHTV AGKHQDLKYISPEIMASVLNGKFAN LIKEFVIIDCRYPYEYEGGHIKGAV NLHMEEEVEDFLLKKPIVPTDGKRV IVVFHCEFSSERGPRMCRYVRERDR LGNEYPKLHYPELYVLKGGYKEFFM KCQSYCEPPSYRPMHHEDFKEDLKK FRTKSRTWAGEKSKREMYSRLKKLT RTRPLEQKLISEEDLAANDILDYKD DDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CDC25A cell division cycle 25A [ Homo sapiens (human) ]
• Official Symbol: CDC25A
• Synonyms: CDC25A; cell division cycle 25 homolog A (S. pombe); cell division cycle 25 homolog A (S. cerevisiae), cell division cycle 25A; M-phase inducer phosphatase 1; CDC25A2-CAG isoform; cell division cycle 25A; dual specificity phosphatase CDC25A; CDC25A2
• Gene ID: 993
• mRNA Refseq: NM_001789
• Protein Refseq: NP_001780
• MIM: 116947
• UniProt ID: P30304