Recombinant Human CDC42BPB Protein, GST-Tagged

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Recombinant Human CDC42BPB Protein, GST-Tagged

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Cat.No. : CDC42BPB-0940H
Product Overview : Human CDC42BPB (NP_006026.3, 1 a.a. - 473 a.a.) partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells. cells.
Description : This gene encodes a member of the serine/threonine protein kinase family. The encoded protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization. [provided by RefSeq, Jul 2008]
Source : Insect Cell
Species : Human
Tag : GST
Form : Liquid
Bio-activity : The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Substrate: DAPK1tide. ATP: 100 μM.
Molecular Mass : 82 kDa
Purity : 61 % by SDS-PAGE/CBB staining
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)
Quality Control Testing :

Loading 1 μg protein in SDS-PAGE

Result of activity analysis :

Gene Name : CDC42BPB CDC42 binding protein kinase beta (DMPK-like) [ Homo sapiens ]
Official Symbol : CDC42BPB
Synonyms : CDC42BPB; CDC42 binding protein kinase beta (DMPK-like); CDC42 binding protein kinase beta (DMPK like); serine/threonine-protein kinase MRCK beta; KIAA1124; MRCKB; MRCK beta; CDC42BP-beta; DMPK-like beta; myotonic dystrophy protein kinase-like beta; CDC42-binding protein kinase beta (DMPK-like); myotonic dystrophy kinase-related CDC42-binding kinase beta; FLJ37601; FLJ44730; DKFZp686H1431;
Gene ID : 9578
mRNA Refseq : NM_006035
Protein Refseq : NP_006026
MIM : 614062
UniProt ID : Q9Y5S2

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