Active Recombinant Human CDC42BPB Protein, GST-Tagged

• Cat.No.: CDC42BPB-0940H
• Product Overview: Human CDC42BPB (NP_006026.3, 1 a.a. - 473 a.a.) partial recombinant protein with GST tag expressed in Baculovirus infected Sf21 cells. cells.

Specification

• Description: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization. [provided by RefSeq, Jul 2008]
• Source: Insect Cell
• Species: Human
• Tag: GST
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg (or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
  Substrate: DAPK1tide. ATP: 100 μM.
• Molecular Mass: 82 kDa
• Purity: 61 % by SDS-PAGE/CBB staining
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.05% Brij35, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: CDC42BPB CDC42 binding protein kinase beta (DMPK-like) [ Homo sapiens ]
• Official Symbol: CDC42BPB
• Synonyms: CDC42BPB; CDC42 binding protein kinase beta (DMPK-like); CDC42 binding protein kinase beta (DMPK like); serine/threonine-protein kinase MRCK beta; KIAA1124; MRCKB; MRCK beta; CDC42BP-beta; DMPK-like beta; myotonic dystrophy protein kinase-like beta; CDC42-binding protein kinase beta (DMPK-like); myotonic dystrophy kinase-related CDC42-binding kinase beta; FLJ37601; FLJ44730; DKFZp686H1431
• Gene ID: 9578
• mRNA Refseq: NM_006035
• Protein Refseq: NP_006026
• MIM: 614062
• UniProt ID: Q9Y5S2