Recombinant Human CDH11 protein, His & GST-tagged

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Recombinant Human CDH11 protein, His & GST-tagged

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Cat.No. : CDH11-1538H
Product Overview : Recombinant Human CDH11 aa. (Val374~Cys595) fused with N-terminal His & GST tag was produced in E. coli cells.
Description : This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance.
Source : E. coli
Species : Human
Tag : His & GST
Form : Freeze-dried powder
Molecular Mass : 55kDa as determined by SDS-PAGE reducing conditions.
Protein length : Val374~Cys595
Endotoxin : <1.0EU per 1µg (determined by the LAL method).
Purity : >95%
Characteristic : The isoelectric point is 5.8.
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200µg/mL
Storage buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
SDS-PAGE :

Gene Name : CDH11 cadherin 11 [ Homo sapiens (human) ]
Official Symbol : CDH11
Synonyms : OB; CAD11; CDHOB; OSF-4; cadherin 11, type 2, OB-cadherin (osteoblast); cadherin-11
Gene ID : 1009
mRNA Refseq : NM_001308392.1
Protein Refseq : NP_001295321.1
UniProt ID : P55287

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