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Recombinant Human CDH11 protein, His & GST-tagged
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Cat.No. : | CDH11-1538H |
Product Overview : | Recombinant Human CDH11 aa. (Val374~Cys595) fused with N-terminal His & GST tag was produced in E. coli cells. |
Description : | This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. |
Source : | E. coli |
Species : | Human |
Tag : | His & GST |
Form : | Freeze-dried powder |
Molecular Mass : | 55kDa as determined by SDS-PAGE reducing conditions. |
Protein length : | Val374~Cys595 |
Endotoxin : | <1.0EU per 1µg (determined by the LAL method). |
Purity : | >95% |
Characteristic : | The isoelectric point is 5.8. |
Applications : | Positive Control; Immunogen; SDS-PAGE; WB. |
Stability : | The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition. |
Storage : | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
Concentration : | 200µg/mL |
Storage buffer : | 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. |
Reconstitution : | Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex. |
SDS-PAGE : |
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Gene Name : | CDH11 cadherin 11 [ Homo sapiens (human) ] |
Official Symbol : | CDH11 |
Synonyms : | OB; CAD11; CDHOB; OSF-4; cadherin 11, type 2, OB-cadherin (osteoblast); cadherin-11 |
Gene ID : | 1009 |
mRNA Refseq : | NM_001308392.1 |
Protein Refseq : | NP_001295321.1 |
UniProt ID : | P55287 |
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◆ Lysates
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