Active Recombinant Human CDH4 protein, His-tagged

• Cat.No.: CDH4-601H
• Product Overview: Active Recombinant Human CDH4 protein(NP_001785.2) (Met 1-Ala 734) was expressed in HEK293 with a fused polyhistidine tag at the C-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-734 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. Human CAD4 immobilized at 0.8 μg/ml, 100 μl/well will mediate >20% C6 cell adhesion.
• Molecular Mass: The recombinant pro form of human CAD4 comprises 725 amino acids and predicts a molecular mass of 80 kDa. As a result of glycosylation, rhCAD4 migrates as an approximately 90-100 kDa protein in SDS-PAGE under reducing conditions.
• Endotoxin: < 1 EU/µg as determined by LAL test.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CDH4 cadherin 4, type 1, R-cadherin (retinal) [ Homo sapiens ]
• Official Symbol: CDH4
• Synonyms: CDH4; cadherin 4, type 1, R-cadherin (retinal); cadherin-4; R Cadherin; R-CAD; R-cadherin; retinal cadherin; cadherin 4, type 1, preproprotein; CAD4; RCAD; FLJ22202; FLJ40547; MGC126700; MGC138355
• Gene ID: 1002
• mRNA Refseq: NM_001252338
• Protein Refseq: NP_001239267
• MIM: 603006
• UniProt ID: P55283