"CDK1/CCNE1" Related Products

Recombinant Human CDK1 & CCNE1 Protein, GST & His-tagged

Cat.No.: CDK1 & CCNE1-1248H
Product Overview: Recombinant human CDC2 (NP_001777.1) (Met1-Met297) was expressed with a GST tag at the N-terminus, constructed the plasmid 1; Recombinant human CCNE1 (NP_001229.1) (Met1-Ala410) was expressed with a polyhistidine tag at the N-terminus, constructed the pla
Source: Baculovirus-Insect cells
Species: Human
Tag: GST & His
Predicted N Terminal: Met & His
Form: Supplied as sterile 20 mM Tris, 500 mM NaCl, 2 mM GSH, 10 % glycerol, pH 8.0.
Bio-activity: The specific activity was determined to be 150 nmol/min/mg using Histone H1 as substrate.
Molecular Mass: The recombinant heterotrimer of human CDC2 & CCNE1 comprises 950 (522+428) amino acids and has a calculated molecular mass of 109.7 (60.4+49.3) kDa.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Gene Name: CDK1 cyclin-dependent kinase 1 [ Homo sapiens ]
Official Symbol: CDK1
Synonyms: CDC2; CDC28A; P34CDC2; cell cycle controller CDC2; cell division control protein 2 homolog; cell division cycle 2, G1 to S and G2 to M; cell division protein kinase 1; p34 protein kinase
Gene ID: 983
mRNA Refseq: NM_001170406
Protein Refseq: NP_001163877
MIM: 116940
UniProt ID:
Chromosome Location: 10q21.1
Pathway: APC/C-mediated degradation of cell cycle proteins, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Centrosome maturation, organism-specific biosystem
Function: ATP binding; RNA polymerase II carboxy-terminal domain kinase activity; cyclin binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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