Active Recombinant Human CDK2 Protein, GST-Tagged

• Cat.No.: CDK2-1003H
• Product Overview: Human CDK2 (NP_001789.2, 1 a.a. - 298 a.a.) and CCNA2 (NP_001228.1, 1 a.a. - 432 a.a.) full-length recombinant protein with with GST tag expressed in baculovirus infected Sf21 cells.

Specification

• Description: This gene encodes a member of a family of serine/threonine protein kinases that participate in cell cycle regulation. The encoded protein is the catalytic subunit of the cyclin-dependent protein kinase complex, which regulates progression through the cell cycle. Activity of this protein is especially critical during the G1 to S phase transition. This protein associates with and regulated by other subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A), and p27Kip1 (CDKN1B). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
• Source: Insect Cell
• Species: Human
• Tag: GST
• Form: Liquid
• Bio-activity: The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorescence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip 3000.
  Substrate: Histone H1 peptide. ATP: 100 μM.
• Molecular Mass: 61 kDa
• Purity: 91 % by SDS-PAGE/CBB staining
• Applications: Functional Study
  SDS-PAGE
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: In 50 mM Tris-HCl, 150 mM NaCl, pH 7.5 (0.1% CHAPS, 1 mM DTT, 10% glycerol)

Gene Information

• Gene Name: CDK2 cyclin-dependent kinase 2 [ Homo sapiens ]
• Official Symbol: CDK2
• Synonyms: CDK2; cyclin-dependent kinase 2; p33 protein kinase; cell devision kinase 2; cdc2-related protein kinase; cell division protein kinase 2; p33(CDK2)
• Gene ID: 1017
• mRNA Refseq: NM_001798
• Protein Refseq: NP_001789
• MIM: 116953
• UniProt ID: P24941