Recombinant Human CDK6/CCND2 Protein (Full length), N-GST-tagged

• Cat.No.: CDK6-27H
• Product Overview: Recombinant full-length human CDK6 and CyclinD2 were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag on both proteins.

Specification

• Description: CDK6 is a member of the cyclin-dependent family of protein kinases that are important regulators of cell cycle progression. CDK6 activity is regulated by the D-type cyclins and members of the INK4 family of CDK inhibitors. The CDK6 kinase activity is detected in mid-G1 phase of the cell cycle and is responsible for the phosphorylation and regulation of the activity of tumor suppressor protein Rb. Although CDK6 and CDK4 can both phosphorylate multiple residues in the Rb protein, they do so with different residue selectivities in vitro; CDK6 phosphorylates Thr821 while CDK4 phosphorylates Thr826 on Rb protein.
• Source: Sf9 insect cells
• Species: Human
• Tag: N-GST
• Molecular Mass: CDK6 Observed MW: ~66 kDa.
  CDK6 Calculated MW: ~63 kDa.
  CyclinD2 Observed MW:: ~60 kDa.
  CyclinD2 Calculated MW: ~59 kDa.
• Protein Length: Full Length
• Purity: The purity of CDK6/CyclinD2 was determined to be > 90 % by densitometry.
• Stability: One year at -70 centigrade from date of shipment.
• Storage: Store product at -70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.2 μg/μL
• Storage Buffer: Recombinant protein stored in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 25% glycerol.
• Shipping: Dry ice

Gene Information

• Gene Name: CDK6 cyclin-dependent kinase 6 [ Homo sapiens (human) ]
• Official Symbol: CDK6
• Synonyms: CDK6; cyclin-dependent kinase 6; PLSTIRE; cell division protein kinase 6; serine/threonine-protein kinase PLSTIRE; MGC59692
• Gene ID: 1021
• mRNA Refseq: NM_001259
• Protein Refseq: NP_001250
• MIM: 603368
• UniProt ID: Q00534