Active Recombinant Human CEACAM3 protein, His-tagged

• Cat.No.: CEACAM3-3942H
• Product Overview: Recombinant Human CEACAM3 protein(Met1-Gly155), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1-155 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human CEACAM3 at 1μg/mL (100 μL/well) can bind CEACAM3 Mouse mAb with a linear range of 0.1-2.7 ng/mL.
• Molecular Mass: 17.62 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 90 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: CEACAM3 carcinoembryonic antigen-related cell adhesion molecule 3 [ Homo sapiens ]
• Official Symbol: CEACAM3
• Synonyms: CEACAM3; carcinoembryonic antigen-related cell adhesion molecule 3; CGM1; CD66d; CD66d antigen; carcinoembryonic antigen CGM1; nonspecific cross-reacting antigen; carcinoembryonic antigen gene family member 1; CEA; W264; W282; CD66D; MGC119875
• Gene ID: 1084
• mRNA Refseq: NM_001815
• Protein Refseq: NP_001806
• MIM: 609142
• UniProt ID: P40198

Case Study

Case 1: Kuiper JWP, et al. J Cell Sci. 2023

CEACAM3 helps immune cells engulf specific bacteria without opsonins by activating Rac, a key player in cell structure changes. Using a CRISPR/Cas9 screen, researchers identified CYRI-B as a protein that hinders this phagocytosis process. Cells without CYRI-B showed enhanced engulfing activity, which reversed when CYRI-B was restored. This highlights CYRI-B's role as a negative regulator in the CEACAM3-mediated bacteria capture pathway.

Fig1. CEACAM3-mediated phagocytosis was determined by flow cytometry.

Fig2. CEACAM3-mediated phagocytosis of Ngo Opa52 or non-opaque gonococci (Ngo Opa−) by the complemented cell lines from B was quantified using gentamicin protection assays.

Case 2: Pils S, et al. PLoS One. 2012

CEACAM3 helps granulocytes recognize and ingest specific bacteria without opsonins through a sequence that gets activated by Src kinases. This activation triggers Rac, leading to actin changes necessary for engulfing bacteria. Our research found that proteins Nck1 and Nck2 bind to phosphorylated CEACAM3, aiding this process. Disrupting Nck1 or Nck2 hampers bacterial intake by affecting actin rearrangements facilitated by the WAVE2 complex.

Fig1. 293 cells were co-transfected with CEACAM3 WT-GFP together with myc-WAVE2 ΔVCA or the empty vector.

Fig2. 293 cells were co-transfected with GFP-WAVE2, myc-Nck1, CEACAM3 WT-HA and vSrc as indicated.

Application

CEACAM3 is a vital protein that helps our immune system recognize and gobble up pathogens. In research, scientists use recombinant CEACAM3 to dive into how it aids the innate immune system, especially in getting rid of harmful bugs. It's known to work with certain molecules to kickstart processes that capture and destroy these pathogens. CEACAM3 also plays a key role in how cells stick together and communicate, which is essential for proper tissue development and cellular interactions. In the realm of cancer, CEACAM3 shows up in unusual ways in some tumors, possibly influencing how they grow and spread. Researchers explore its role in the tumor environment to see if it could serve as a marker or target for cancer treatment. This protein is also a big player in drug research, helping screen for substances that might tweak immune responses, paving the way for new therapies. It's used in various lab techniques to check its levels in different cells and tissues. On the industrial side, recombinant CEACAM3 is crafted using genetic engineering, ensuring it's pure and fit for research and diagnostics. It's especially handy in developing diagnostic tools to assess CEACAM3 levels in health conditions. Its immune role also positions it for the creation of targeted drugs. In studying infectious diseases, CEACAM3 helps explain how the body deals with bacterial threats, potentially leading to new antibacterial treatments. Overall, recombinant CEACAM3 is a multifaceted tool in areas like immunology and cancer biology, crucial for understanding how cells stick and how immune defenses work.

Fig1. Schematic overview of the negative regulation of CEACAM3. (Johannes W P Kuiper, 2024)