Recombinant Human CEACAM5 cell lysate

Cat.No. : CEACAM5-2238HCL
Product Overview : Human CEACAM5 / CD66e derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
  • Specification
  • Gene Information
  • Related Products
  • Download
Source : Human cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : CEACAM5 carcinoembryonic antigen-related cell adhesion molecule 5 [ Homo sapiens ]
Official Symbol : CEACAM5
Synonyms : CEACAM5; carcinoembryonic antigen-related cell adhesion molecule 5; CEA; CD66e; meconium antigen 100; DKFZp781M2392;
Gene ID : 1048
mRNA Refseq : NM_004363
Protein Refseq : NP_004354
MIM : 114890
UniProt ID : P06731
Chromosome Location : 19q13.1-q13.2

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Inquiry

  • Q&As
  • Reviews

Q&As (5)

Ask a question
Can CEACAM5 levels be used to detect cancer in its early stages? 03/28/2021

CEACAM5 can be a helpful marker, but it is not typically used for early cancer detection.

Are there any limitations to using CEACAM5 as a cancer marker? 04/07/2018

CEACAM5 is not specific to a single cancer type and may also be elevated in non-cancerous conditions.

What other medical conditions or diseases can cause an increase in CEACAM5 levels? 02/14/2018

Conditions like hepatitis, pancreatitis, and some non-cancerous gastrointestinal diseases can also elevate CEACAM5 levels.

How is CEACAM5 protein used in cancer treatment monitoring? 12/27/2016

CEACAM5 levels are monitored to assess the effectiveness of cancer treatment and to detect cancer recurrence.

Can CEACAM5 levels vary between different individuals, and if so, why? 12/03/2016

Yes, CEACAM5 levels can vary between individuals due to genetic factors and other individual-specific variables.

Customer Reviews (3)

Write a review
Reviews
03/03/2020

    CEACAM5 protein has exhibited remarkable performance in Western Blotting experiments.

    08/06/2019

      This enhanced visibility and distinctness of the protein bands greatly aided in the accurate analysis and interpretation of my Western Blotting results.

      09/23/2018

        Its reliability, versatility, and ability to deliver clear and reliable results make it a top choice for researchers in need of a high-quality protein for their experiments.

        Ask a Question for All CEACAM5 Products

        Required fields are marked with *

        My Review for All CEACAM5 Products

        Required fields are marked with *

        0

        Inquiry Basket

        cartIcon
        logo

        FOLLOW US

        Terms and Conditions        Privacy Policy

        Copyright © 2024 Creative BioMart. All Rights Reserved.

        Contact Us

        • /

        Stay Updated on the Latest Bioscience Trends