Recombinant Human CEACAM5 cell lysate
Cat.No. : | CEACAM5-2238HCL |
Product Overview : | Human CEACAM5 / CD66e derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls |
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Source : | Human cells |
Species : | Human |
Preparation method : | Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized. |
Lysis buffer : | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Quality control Testing : | 12.5% SDS-PAGE Stained with Coomassie Blue |
Recommended Usage : | 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating. |
Stability : | Samples are stable for up to twelve months from date of receipt at -80°C |
Storage Buffer : | 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF |
Storage Instruction : | Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes. |
Tag : | Non |
Gene Name : | CEACAM5 carcinoembryonic antigen-related cell adhesion molecule 5 [ Homo sapiens ] |
Official Symbol : | CEACAM5 |
Synonyms : | CEACAM5; carcinoembryonic antigen-related cell adhesion molecule 5; CEA; CD66e; meconium antigen 100; DKFZp781M2392; |
Gene ID : | 1048 |
mRNA Refseq : | NM_004363 |
Protein Refseq : | NP_004354 |
MIM : | 114890 |
UniProt ID : | P06731 |
Chromosome Location : | 19q13.1-q13.2 |
Products Types
◆ Recombinant Protein | ||
CEACAM5-733H | Recombinant Human CEACAM5 Protein, His-tagged | +Inquiry |
CEACAM5-732H | Recombinant Human CEACAM5 Protein, His-tagged | +Inquiry |
CEACAM5-631R | Recombinant Rhesus Macaque CEACAM5 Protein, His (Fc)-Avi-tagged | +Inquiry |
Ceacam5-002M | Active Recombinant Mouse Ceacam5 Protein, Fc-tagged | +Inquiry |
CEACAM5-494H | Recombinant Human CEACAM5 protein(Met1-Ala685), hFc-tagged | +Inquiry |
◆ Native Protein | ||
CEACAM5-27803TH | Native Human CEACAM5 | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Customer Reviews (3)
Write a reviewCEACAM5 protein has exhibited remarkable performance in Western Blotting experiments.
This enhanced visibility and distinctness of the protein bands greatly aided in the accurate analysis and interpretation of my Western Blotting results.
Its reliability, versatility, and ability to deliver clear and reliable results make it a top choice for researchers in need of a high-quality protein for their experiments.
Q&As (5)
Ask a questionCEACAM5 can be a helpful marker, but it is not typically used for early cancer detection.
CEACAM5 is not specific to a single cancer type and may also be elevated in non-cancerous conditions.
Conditions like hepatitis, pancreatitis, and some non-cancerous gastrointestinal diseases can also elevate CEACAM5 levels.
CEACAM5 levels are monitored to assess the effectiveness of cancer treatment and to detect cancer recurrence.
Yes, CEACAM5 levels can vary between individuals due to genetic factors and other individual-specific variables.
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