Recombinant Human CEACAM5 Protein (Met1-Ala685), His-tagged, Biotinylated

• Cat.No.: CEACAM5-495H
• Product Overview: Recombinant Human CEACAM5 Protein (NP_004354.2) (Met1-Ala685) was produced by HEK293 Cells expression system. This protein was fused with a polyhistidine tag at the C-terminus. The purified protein was biotinylated in vitro.

Specification

• Description: CEACAM5, also known as CEA or D66e, belongs to the large CEACAM subfamily of immunoglobulin superfamily. CEACAM5 is expressed primarily by epithelial cells, and is synthesized as a glycoprotein with a MW of 18 kDa comprising 6% carbohydrate. CEACAM5 contains one Ig-like V-type domain at the N-terminus, followed by six Ig-like C2-type domain and a GPI anchor, and exists as a homodimer. CEACAM5 and CEACAM6 are overexpressed in many cancers and are associated with adhesion and invasion. CEACAM5 can mediate cell-cell adhesion through homotypic and heterotypic interactions. It functions as a homotypic intercellular adhesion molecule and serves as a widely used tumor marker, since it is expressed at higher levels in tumorous tissues than in corresponding normal tissues. CEACAM5 has also been shown to contribute to tumorigenicity by inhibiting cellular differentiation. In addition, CEACAM5 is identified as the host receptor for the Dr family of adhesins of E.Coli, and the binding of E.coli Dr adhesins leads to dissociation of the CEACAM5 homodimer.
• Source: HEK293
• Species: Human
• Tag: His
• Predicted N Terminal: Lys 35
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The secreted recombinant human CEACAM5 consists of 662 amino acids and has a predicted molecular mass of 72.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhCEACAM5 is approximately 100-110 kDa due to glycosylation.
• Protein length: Met1-Ala685
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade.
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4℃ before opening to recover the entire contents.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
  Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: CEACAM5 carcinoembryonic antigen-related cell adhesion molecule 5 [ Homo sapiens ]
• Official Symbol: CEACAM5
• Synonyms: CEACAM5; CEA; CD66e; meconium antigen 100; DKFZp781M2392
• Gene ID: 1048
• mRNA Refseq: NM_004363
• Protein Refseq: NP_004354
• MIM: 114890
• UniProt ID: P06731

Reviews

03/03/2020

CEACAM5 protein has exhibited remarkable performance in Western Blotting experiments.

08/06/2019

This enhanced visibility and distinctness of the protein bands greatly aided in the accurate analysis and interpretation of my Western Blotting results.

09/23/2018

Its reliability, versatility, and ability to deliver clear and reliable results make it a top choice for researchers in need of a high-quality protein for their experiments.

Q&As

Can CEACAM5 levels be used to detect cancer in its early stages?

CEACAM5 can be a helpful marker, but it is not typically used for early cancer detection.

Are there any limitations to using CEACAM5 as a cancer marker?

CEACAM5 is not specific to a single cancer type and may also be elevated in non-cancerous conditions.

What other medical conditions or diseases can cause an increase in CEACAM5 levels?

Conditions like hepatitis, pancreatitis, and some non-cancerous gastrointestinal diseases can also elevate CEACAM5 levels.

How is CEACAM5 protein used in cancer treatment monitoring?

CEACAM5 levels are monitored to assess the effectiveness of cancer treatment and to detect cancer recurrence.

Can CEACAM5 levels vary between different individuals, and if so, why?

Yes, CEACAM5 levels can vary between individuals due to genetic factors and other individual-specific variables.