Recombinant Human CENPH, His-tagged

• Cat.No.: CENPH-3644H
• Product Overview: Recombinant human CENPH protein, fused to His-tag at N-terminus, was expressed in E.coli and denatured using detergent during a conventional chromatography purification process (136-247aa).

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: CENPH, also known as centromere protein H, belongs to the centromere protein H family. This protein is component of the CENPA-NAC (nucleosome-associated) complex that plays a central role in assembly of kinetochore proteins. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
• Molecular Weight: 15.5 kDa (133aa)
• Purity: > 90% by SDS - PAGE
• Concentration: 1.0 mg/ml (determined by UV spectroscopy at 280 nm)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MLNKLIMKSQ QESWDLEEKL LDIRKKRLQL KQASESKLLE IQTEKNKQKI DLDSMENSER IKIIRQNLQM EIKITTVIQH VFQNLILGSK VNWAEDPALK EIVLQLEKNV DMM
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Cell Cycle, Mitotic; Chromosome Maintenance; DNA Replication; Deposition of New CENPA-containing Nucleosomes at the Centromere; M Phase; Mitotic M-M/G1 phases; Mitotic Prometaphase; Nucleosome assembly

Gene Information

• Gene Name: CENPH centromere protein H [ Homo sapiens ]
• Official Symbol: CENPH
• Synonyms: CENPH; ICEN35; NNF1; PMF1; centromere protein H; centromere protein H; CENP-H; OTTHUMP00000123477; OTTHUMP00000221978; OTTHUMP00000221979; kinetochore protein CENP-H; interphase centromere complex protein 35
• Gene ID: 64946
• mRNA Refseq: NM_022909
• Protein Refseq: NP_075060
• MIM: 605607
• UniProt ID: Q9H3R5
• Chromosome Location: 5p15.2
• Function: kinetochore binding; protein binding