Recombinant Human CFH cell lysate

• Cat.No.: CFH-2409HCL
• Product Overview: Human CFH / Complement Factor H derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Human cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: CFH complement factor H [ Homo sapiens ]
• Official Symbol: CFH
• Synonyms: CFH; complement factor H; H factor 1 (complement) , HF, HF1, HF2; age related maculopathy susceptibility 1; ARMS1; beta 1H; FHL1; H factor 2 (complement); HUS; beta-1H; factor H; factor H-like 1; beta-1-H-globulin; H factor 1 (complement); adrenomedullin binding protein; complement factor H, isoform b; age-related maculopathy susceptibility 1; FH; HF; HF1; HF2; AHUS1; AMBP1; ARMD4; CFHL3; MGC88246
• Gene ID: 3075
• mRNA Refseq: NM_000186
• Protein Refseq: NP_000177
• MIM: 134370
• UniProt ID: P08603
• Chromosome Location: 1q32
• Pathway: Complement and coagulation cascades, organism-specific biosystem; Complement and coagulation cascades, conserved biosystem; Complement cascade, organism-specific biosystem; Immune System, organism-specific biosystem; Innate Immune System, organism-specific biosystem; Regulation of Complement cascade, organism-specific biosystem; Staphylococcus aureus infection, organism-specific biosystem

Reviews

11/16/2020

Exceptional protein expression optimization support, highly appreciated assistance.

07/31/2020

Precise protein quantification, ensures data accuracy in our research.

03/10/2018

Timely antibody validation, an invaluable asset for our laboratory.

Q&As

What is the impact of CFH mutations on the risk of developing atypical hemolytic uremic syndrome (aHUS)?

Mutations in CFH increase the risk of aHUS, affecting complement regulation and leading to kidney damage.

How does CFH (Complement Factor H) regulate the complement system and prevent overactivation?

CFH controls the complement system, preventing its overactivation and protecting against immune damage.

How does CFH influence the immune response to bacterial infections?

CFH modulates the immune response to bacterial infections, balancing defense mechanisms.

How does CFH deficiency contribute to the development of age-related macular degeneration (AMD)?

CFH deficiency is linked to AMD, due to impaired regulation of the complement pathway in the retina.

What role does CFH play in protecting host cells from complement-mediated damage?

CFH shields host cells from complement-mediated injury, maintaining tissue integrity.

What are the mechanisms by which CFH modulates inflammation and immune homeostasis?

CFH regulates inflammation and maintains immune balance, crucial for preventing excessive immune responses.

How do alterations in CFH expression or function affect renal function and the development of kidney diseases?

Changes in CFH function can impact kidney health, potentially leading to renal diseases due to altered complement activity.