Recombinant Human Charcot-Leyden Crystal Protein, His-tagged

• Cat.No.: CLC-984H
• Product Overview: Recombinant human CLC protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques(1-142aa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Eosinophil lysophospholipase (CLC), also known as Galectin 10, act on biological membranes to regulate the multifunctional lysophospholipids. CLC is a lysophospholipase expressed in eosinophils and basophils. It hydrolyzes lysophosphatidylcholine to glycerophosphocholine and a free fatty acid. This protein may possess carbohydrate or IgE-binding activities. It is both structurally and functionally related to the galectin family of beta-galactoside binding proteins. It may be associated with inflammation and some myeloid leukemias.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,10% glycerol, 0.1M NaCl
• Molecular Weight: 18.6 kDa (162aa) confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay )
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MSLLPVPYTE AASLSTGSTV TIKGRPLACF LNEPYLQVDF HTEMKEESDI VFHFQVCFGR RVVMNSREYGAWKQQVESKN MPFQDGQEFE LSISVLPDKY QVMVNGQSSY TFDHRIKPEA VKMVQVWRDI SLTKFNVSYL KR
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CLC Charcot-Leyden crystal protein [ Homo sapiens ]
• Synonyms: CLC; GAL10; Gal-10; LGALS10; LGALS10A; MGC149659; LPPL_HUMAN; galectin-10; eosinophil lysophospholipase; galectin 10; lysolecithinacylhydrolase; NP_001819.2; EC 3.1.1.5
• Gene ID: 1178
• mRNA Refseq: NM_001828
• Protein Refseq: NP_001819
• MIM: 153310
• UniProt ID: Q05315
• Chromosome Location: 19q13.1
• Function: hydrolase activity; lysophospholipase activity; lysophospholipase activity