Recombinant Human Chemokine (C-C motif) Ligand 3, His-tagged CCL3-169H

Recombinant Human Chemokine (C-C motif) Ligand 3, His-tagged

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Recombinant Human Chemokine (C-C motif) Ligand 3, His-tagged

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Cat.No. : CCL3-169H Optional Service: Optional requirements on this protein
Product Overview : Recombinant human CCL3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer(24-92aa).
Description : CCL3, also known as, macrophage inflammatory protein-1alpha (MIP-1alpha), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This protein has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1beta and RANTES. It acts as an inhibitor of different strains of HIV-1, HIV-2 and SIV infection in a dose-dependent manner.
Form : Liquid. In 10mM Sodium Citrate ( pH3.5) containing 10% glycerol
Molecular Weight : 10.0 kDa (90aa), confirmed by MALDI-TOF(Molecular weight on SDS-PAGE will appear higher)
Purity : > 90% by SDS - PAGE
Concentration : 0.25 mg/ml (determined by Bradford assay)
Endotoxin Level : < 1.0 EU per 1 μg of protein (determined by LAL method )
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MSLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Figure :

Gene Name : CCL3 chemokine (C-C motif) ligand 3 [ Homo sapiens ]
Synonyms : CCL3; MIP1A; SCYA3; G0S19-1; LD78ALPHA; MIP-1-alpha; C-C motif chemokine 3; SIS-beta; PAT 464.1; G0/G1 switch regulatory protein 19-1; macrophage inflammatory protein 1-alpha;tonsillar lymphocyte LD78 alpha protein;small inducible cytokine A3 (homologous to mouse Mip-1a)
Gene ID : 6348
mRNA Refseq : NM_002983
Protein Refseq : NP_002974
MIM : 182283
UniProt ID : P10147
Chromosome Location : 17q12
Function : chemoattractant activity; chemokine activity; signal transducer activity
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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