Recombinant Human CHKA Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CHKA-5412H
• Product Overview: CHKA MS Standard C13 and N15-labeled recombinant protein (NP_997634) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. The protein encoded by this gene is the initial enzyme in the sequence and may play a regulatory role. The encoded protein also catalyzes the phosphorylation of ethanolamine. Two transcript variants encoding different isoforms have been found for this gene.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 50 kDa
• AA Sequence: MKTKFCTGGEAEPSPLGLLLSCGSG SAAPAPGVGQQRDAASDLESKQLGG QQPPLALPPPPPLPLPLPLPQPPPP QPPADEQPEPRTRRRAYLWCKEFLP GAWRGLREDEFHISVIRGGLSNMLF QCSLPDTTATLGDEPRKVLLRLYGA ILQVGAEAMVLESVMFAILAERSLG PKLYGIFPQGRLEQFIPSRRLDTEE LGLPDISAEIAEKMATFHGMKMPFN KEPKWLFGTMEKYLKEVLRIKFTEE SRIKKLHKLLSYNLPLELENLRSLL ESTPSPVVFCHNDCQEGNILLLEGR ENSEKQKLMLIDFEYSSYNYRGFDI GNHFCEWMYDYSYEKYPFFRANIRK YPTKKQQLHFISSYLPAFQNDFENL STEEKSIIKEEMLLEVNRFALASHF LWGQWSIVQAKISSIEFGYMDYAQA RFDAYFHQKRKLGVTRTRPLEQKLI SEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CHKA choline kinase alpha [ Homo sapiens (human) ]
• Official Symbol: CHKA
• Synonyms: CHKA; choline kinase alpha; CHK, choline kinase; CKI; CHETK-alpha; ethanolamine kinase; CK; EK; CHK
• Gene ID: 1119
• mRNA Refseq: NM_212469
• Protein Refseq: NP_997634
• MIM: 118491
• UniProt ID: P35790