Recombinant Human CHRNB3 Protein, GST-Tagged

• Cat.No.: CHRNB3-1288H
• Product Overview: Human CHRNB3 full-length ORF (NP_000740.1, 1 a.a. - 458 a.a.) recombinant protein with GST-tag at N-terminal.

Specification

• Description: The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are (hetero)pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of nAChRs are encoded by separate genes and have different primary structure. There are several subtypes of neuronal nAChRs that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if, like muscle alpha-1 (MIM 100690), they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits (Groot Kormelink and Luyten, 1997 [PubMed 9009220]). Elliott et al. (1996) [PubMed 8906617] stated that the proposed structure for each subunit is a conserved N-terminal extracellular domain followed by 3 conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region.[supplied by OMIM, Apr 2010]
• Source: Wheat Germ
• Species: Human
• Tag: GST
• Molecular Mass: 79.1 kDa
• AA Sequence: MLPDFMLVLIVLGIPSSATTGFNSI AENEDALLRHLFQGYQKWVRPVLHS NDTIKVYFGLKISQLVDVDEKNQLM TTNVWLKQEWTDHKLRWNPDDYGGI HSIKVPSESLWLPDIVLFENADGRF EGSLMTKVIVKSNGTVVWTPPASYK SSCTMDVTFFPFDRQNCSMKFGSWT YDGTMVDLILINENVDRKDFFDNGE WEILNAKGMKGNRRDGVYSYPFITY SFVLRRLPLFYTLFLIIPCLGLSFL TVLVFYLPSDEGEKLSLSTSVLVSL TVFLLVIEEIIPSSSKVIPLIGEYL LFIMIFVTLSIIVTVFVINVHHRSS STYHPMAPWVKRLFLQKLPKLLCMK DHVDRYSSPEKEESQPVVKGKVLEK KKQKQLSDGEKVLVAFLEKAADSIR YISRHVKKEHFISQVVQDWKFVAQV LDRIFLWLFLIVSVTGSVLIFTPAL KMWLHSYH
• Applications: Enzyme-linked Immunoabsorbent Assay
  Western Blot (Recombinant protein)
  Antibody Production
  Protein Array
• Storage: Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
• Storage Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.

Gene Information

• Gene Name: CHRNB3 cholinergic receptor, nicotinic, beta 3 (neuronal) [ Homo sapiens ]
• Official Symbol: CHRNB3
• Synonyms: CHRNB3; cholinergic receptor, nicotinic, beta 3 (neuronal); cholinergic receptor, nicotinic, beta polypeptide 3; neuronal acetylcholine receptor subunit beta-3; acetylcholine receptor; nicotinic; beta 3 (neuronal); acetylcholine receptor, nicotinic, beta 3 (neuronal); acetylcholine receptor, neuronal nicotinic, beta-3 subunit
• Gene ID: 1142
• mRNA Refseq: NM_000749
• Protein Refseq: NP_000740
• MIM: 118508
• UniProt ID: Q05901