Recombinant Human CLEC11A Protein, Gly19-Phe323, C-6×His tagged

• Cat.No.: CLEC11A-50H
• Product Overview: Recombinant Human CLEC11A Protein, Gly19-Phe323, with an N-terminal Met and a C-terminal 6-His tag was expressed in Mouse myeloma cell line.

Specification

• Description: This gene encodes a member of the C-type lectin superfamily. The encoded protein is a secreted sulfated glycoprotein and functions as a growth factor for primitive hematopoietic progenitor cells. An alternative splice variant has been described but its biological nature has not been determined.
• Source: Mouse myeloma cell line
• Species: Human
• Tag: His
• Protein length: Gly19-Phe323
• Bio-activity: Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c-kit Ligand, Recombinant Mouse Thrombopoietin/Tpo), and Recombinant Mouse Flt-3 Ligand. The ED50 for this effect is 0.12-0.6 μg/mL in the presence of cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.
• Molecular Mass: Predicted Molecular Mass: 34.7 kDa
  SDS-PAGE: 50 kDa, reducing conditions
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• N-terminal Sequence Analysis: Met
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
• References: 1. Mio, H. et al. (1998) Biochem. Biophys. Res. Commun. 249:124.
  2. Bannwarth, S. et al. (1998) J. Biol. Chem. 273:1911.
  3. Hiraoka, A. et al. (1997) Proc. Natl. Acad. Sci. 94:7577.

Gene Information

• Gene Name: CLEC11A C-type lectin domain containing 11A [ Homo sapiens (human) ]
• Official Symbol: CLEC11A
• Synonyms: CLEC11A; C-type lectin domain containing 11A; P47; SCGF; LSLCL; CLECSF3; C-type lectin domain family 11 member A; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 3; C-type lectin superfamily member 3; lymphocyte secreted C-type lectin; lymphocyte secreted long form of C-type lectin; osteolectin; stem cell growth factor; stem cell growth factor; lymphocyte secreted C-type lectin
• Gene ID: 6320
• mRNA Refseq: NM_002975
• Protein Refseq: NP_002966
• MIM: 604713
• UniProt ID: Q9Y240