Recombinant Human CLEC4C protein, His-Avi-tagged, Biotinylated

• Cat.No.: CLEC4C-1363H
• Product Overview: Biotinylated Recombinant Human CLEC4C protein(Q8WTT0-1)(Phe46-Ile213), fused with N-terminal His and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: N-His-Avi
• Protein length: Phe46-Ile213
• Conjugation/Label: Biotin
• Form: Supplied as 0.22μm filtered solution in 20mM Tris, 150mM NaCl, 200mM L-arginine (pH 8.5).
• Bio-activity: Immobilized Anti-BDCA-2 Antibody at 1μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Human BDCA-2, His Tag with the EC50 of 9.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 22.7 kDa. Due to glycosylation, the protein migrates to 33-40 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CLEC4C C-type lectin domain family 4, member C [ Homo sapiens ]
• Official Symbol: CLEC4C
• Synonyms: DLEC; HECL; BDCA2; CD303; CLECSF7; CLECSF11; PRO34150
• Gene ID: 170482
• mRNA Refseq: NM_203503.1
• Protein Refseq: NP_987099.1
• MIM: 606677
• UniProt ID: Q8WTT0