Recombinant Human CLEC4C protein, His-tagged

• Cat.No.: CLEC4C-1368H
• Product Overview: Recombinant Human CLEC4C protein(Q8WTT0-1)(Phe46-Ile213), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: N-His
• Protein Length: Phe46-Ile213
• Tag: N-His
• Form: Supplied as 0.22μm filtered solution in 20mMTris, 300mM NaCl, 200mM L-Arginine PH (pH 8.5).
• Bio-activity: Immobilized Human BDCA-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-BDCA-2 Antibody, hFc Tag with the EC50 of 8.2ng/ml determined by ELISA. The affinity constant of 2.97 nM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 20.93 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC.
• Storage: Valid for 12 months from date of receipt when stored at -80°C.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CLEC4C C-type lectin domain family 4, member C [ Homo sapiens ]
• Official Symbol: CLEC4C
• Synonyms: DLEC; HECL; BDCA2; CD303; CLECSF7; CLECSF11; PRO34150
• Gene ID: 170482
• mRNA Refseq: NM_203503.1
• Protein Refseq: NP_987099.1
• MIM: 606677
• UniProt ID: Q8WTT0