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Recombinant Human CLU Protein, His-tagged

Cat.No.: CLU-11H
Product Overview: Recombinant Human CLU Protein [Asp23-Arg227 (β chain) & Ser228-Glu449 (α chain) ] (NP_001822.3), fused with 6*His tag at the C terminus, was expressed in HEK293.
Source: HEK293
Species: Human
Tag : His
Form: Lyophilized from sterile PBS, pH7.4, 10% trehalose.
Molecular Mass: Recombinant Human CLU Protein has a calculated MW of 50.9 kDa (Asp23-Glu449, α chain 24.5kDa + β chain 27kDa). The recombinant rh CLUS / Clusterin (Asp23-Glu 449) was cleaved into α chain and β chain, which form a heterodimer linked by disulfide bonds. DTT reduced protein migrates as 39 kDa and 40 kDa bands in SDS-PAGE due to glycosylation, corresponding to the cleaved β chain, and α chain respectively.
Protein length: Asp23-Arg227 (β chain) & Ser228-Glu449 (α chain)
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >90% as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: CLU clusterin [ Homo sapiens ]
Official Symbol: CLU
Synonyms: CLU; clusterin; APOJ, CLI, clusterin (complement lysis inhibitor, SP 40,40, sulfated glycoprotein 2, testosterone repressed prostate message 2, apolipoprotein J); apolipoprotein J; complement lysis inhibitor; KUB1; SGP 2; SP 40; sulfated glycoprotein 2; testosterone repressed prostate message 2; TRPM 2; ku70-binding protein 1; aging-associated protein 4; complement cytolysis inhibitor; complement-associated protein SP-40,40; testosterone-repressed prostate message 2; CLI; AAG4; APOJ; SGP2; APO-J; SGP-2; SP-40; TRPM2; TRPM-2; NA1/NA2; MGC24903;
Gene ID: 1191
mRNA Refseq: NM_001831
Protein Refseq: NP_001822
MIM: 185430
UniProt ID: P10909
Chromosome Location: 8p21-p12
Pathway: Hemostasis, organism-specific biosystem; Platelet activation, signaling and aggregation, organism-specific biosystem; Platelet degranulation, organism-specific biosystem; Response to elevated platelet cytosolic Ca2+, organism-specific biosystem; Validated targets of C-MYC transcriptional repression, organism-specific biosystem;
Function: NOT ATPase activity; misfolded protein binding; misfolded protein binding; protein binding; ubiquitin protein ligase binding;

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