Recombinant Human CNDP1 Protein, His-tagged

• Cat.No.: CNDP1-382H
• Product Overview: Recombinant human CNDP1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Description: CNDP1, also known as beta-Ala-His dipeptidase, belongs to the peptidase M20A family. The shortest allelic form (CNDP1 Mannheim) was more common in the absence of nephropathy and was associated with lower serum carnosinase levels. Carnosine inhibited the increased production of fibronectin and collagen type VI in podocytes and the increased production of TGF-beta in mesangial cells. Diabetic patients with the CNDP1 Mannheim variant are less susceptible for nephropathy. Carnosine protects against the adverse effects of high glucose levels on renal cells.
• Form: Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
• Molecular Mass: 54.9kDa (489aa)50-70KDa (SDS-PAGE under reducing conditions.)
• AA Sequence: SPSPPPALLE KVFQYIDLHQ DEFVQTLKEW VAIESDSVQP VPRFRQELFR MMAVAADTLQ RLGARVASVD MGPQQLPDGQ SLPIPPVILA ELGSDPTKGT VCFYGHLDVQ PADRGDGWLT DPYVLTEVDG KLYGRGATDN KGPVLAWINA VSAFRALEQD LPVNIKFIIE GMEEAGSVAL EELVEKEKDR FFSGVDYIVI SDNLWISQRK PAITYGTRGN SYFMVEVKCR DQDFHSGTFG GILHEPMADL VALLGSLVDS SGHILVPGIY DEVVPLTEEE INTYKAIHLD LEEYRNSSRV EKFLFDTKEE ILMHLWRYPS LSIHGIEGAF DEPGTKTVIP GRVIGKFSIR LVPHMNVSAV EKQVTRHLED VFSKRNSSNK MVVSMTLGLH PWIANIDDTQ YLAAKRAIRT VFGTEPDMIR DGSTIPIAKM FQEIVHKSVV LIPLGAVDDG EHSQNEKINR WNYIEGTKLF AAFFLEMAQL HLEHHHHHH
• Endotoxin: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Purity: >95% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm)

Gene Information

• Gene Name: CNDP1 carnosine dipeptidase 1 (metallopeptidase M20 family) [ Homo sapiens ]
• Official Symbol: CNDP1
• Synonyms: CNDP1; carnosine dipeptidase 1 (metallopeptidase M20 family); beta-Ala-His dipeptidase; carnosinase 1; CN1; CPGL2; glutamate carboxypeptidase like protein 2; HsT2308; MGC10825; serum carnosinase; CNDP dipeptidase 1; glutamate carboxypeptidase-like protein 2; MGC102737; MGC142072
• Gene ID: 84735
• mRNA Refseq: NM_032649
• Protein Refseq: NP_116038
• MIM: 609064
• UniProt ID: Q96KN2
• Chromosome Location: 18q22.3
• Pathway: Arginine and proline metabolism, organism-specific biosystem; Arginine and proline metabolism, conserved biosystem; Histidine metabolism, organism-specific biosystem; Histidine metabolism, conserved biosystem; Metabolic pathways, organism-specific biosystem; beta-Alanine metabolism, organism-specific biosystem; beta-Alanine metabolism, conserved biosystem
• Function: carboxypeptidase activity; dipeptidase activity; metal ion binding; metallopeptidase activity; peptidase activity; tripeptidase activity