Recombinant Human COL2A1 protein, His-tagged

• Cat.No.: COL2A1-3929H
• Product Overview: Recombinant Human COL2A1 protein(Asp 1242-Leu 1487)(P02458-2) was expressed in HEK293, with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 1242-1487 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The recombinant human COL2A1 (aa 1242-1487), known as chondrocalcin consists of 265 amino acids and has a calculated molecular mass of 29.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 37 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Publications:
  • Pathogenic Role of Circulating Citrullinated Antigens and Anti-Cyclic Monoclonal Citrullinated Peptide Antibodies in Rheumatoid Arthritis (2021)

Gene Information

• Gene Name: COL2A1 collagen, type II, alpha 1 [ Homo sapiens ]
• Official Symbol: COL2A1
• Synonyms: COL2A1; collagen, type II, alpha 1; collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital), SEDC; collagen alpha-1(II) chain; chondrocalcin; cartilage collagen; alpha-1 type II collagen; collagen II, alpha-1 polypeptide; AOM; ANFH; SEDC; COL11A3; MGC131516
• Gene ID: 1280
• mRNA Refseq: NM_001844
• Protein Refseq: NP_001835
• UniProt ID: P02458