Recombinant Human COL4A4 Protein, His-tagged

• Cat.No.: COL4A4-333H
• Product Overview: Recombinant Human COL4A4(Val1469~Ser1690) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Description: This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. This particular collagen IV subunit, however, is only found in a subset of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Mutations in this gene are associated with type II autosomal recessive Alport syndrome (hereditary glomerulonephropathy) and with familial benign hematuria (thin basement membrane disease). Two transcripts, differing only in their transcription start sites, have been identified for this gene and, as is common for collagen genes, multiple polyadenylation sites are found in the 3' UTR.
• Source: E. coli
• Species: Human
• Tag: His
• Form: 100mMNaHCO3, 500mMNaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Molecular Mass: 28.3kDa
• Protein length: Val1469~Ser1690
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: > 95%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
  If bio-activity of the protein is needed, please check active protein
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Reconstitution: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: COL4A4 collagen type IV alpha 4 chain [ Homo sapiens ]
• Official Symbol: COL4A4
• Synonyms: CA44; Collagen IV, alpha-4 polypeptide; collagen of basement membrane, alpha-4 chain; collagen, type IV, alpha 4
• Gene ID: 1286
• mRNA Refseq: NM_000092
• Protein Refseq: NP_000083
• UniProt ID: P53420