Recombinant Human Complexin 1, His-tagged

• Cat.No.: CPLX1-305H
• Product Overview: Recombinant Complexin 1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (154 aa, 17.1 kDa).

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Complexin1 belongs to a family of SNARE complex binding proteins that were suggested to be either facilitators or inhibitors of vesicle exocytosis. Complexin 1 exhibits reduced Ca2+-triggered fast neurotransmitter release at hippocampal glutamatergic synapses, indicating that this protein is a positive regulator of transmitter release. In contrast, it inhibits SNARE-mediated liposome and cell fusions in vitro, which lead to the hypothesis that it acts as fusion clamps of synaptic exocytosis.
• Formulation: Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.
• Molecular Weight: 17.1 kDa (154 aa) (Real molecular weight on SDS-PAGE will be shift up).
• Purity: > 90% by SDS – PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Endotoxin Level: < 1.0 EU per 1 μg of protein (determined by LAL method).
• Sequence: MGSSHHHHHH SSGLVPRGSH MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA KYAKMEAERE AVRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA IPPGCGDEVE EEDESILDTV IKYLPGPLQD MLKK
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: CPLX1
• Synonyms: CPLX1; complexin 1; CPX1; CPX-I; CPLX1; CPX I; Complexin I; complexin 1 synaphin 2; Synaphin-2.
• Gene ID: 10815
• mRNA Refseq: NM_006651.3
• Protein Refseq: NP_006642.1
• MIM: 605032
• UniProt ID: O14810
• Chromosome Location: 4p16.3
• Pathway: Synaptic Transmission.
• Function: syntaxin binding.