Recombinant Human COMT Protein, His/SUMOpro-tagged

• Cat.No.: COMT-021H
• Product Overview: Active recombinant N-terminal His and SUMOpro tagged full length human COMT protein (2-221) was expressed in E. coli.

Specification

• Description: Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters.
• Source: E. coli
• Species: Human
• Tag: His/SUMOpro
• Molecular Mass: 37.6 kDa
• Protein length: 2-221
• Purity: ≥70% estimated by SDS-PAGE
• Unit Definition: One unit produces 1 nmol/hr of O-methylated products from 3,4-dihydroxyacetophenone (DHAP) with S-adenosyl-L-methionine (SAM) as substrate at pH 7.6, 37 centigrade, measured by absorbance at 344 nm.
• Applications: ELISA, WB, and Enzyme activity assay
• Stability: ≥ 1 year
• Storage: At -80 centigrade.
• Storage Buffer: 50 mM Hepes, pH 8.0, with 500 mM NaCl and 10% glycerol

Gene Information

• Gene Name: COMT catechol-O-methyltransferase [ Homo sapiens (human) ]
• Official Symbol: COMT
• Synonyms: COMT; catechol-O-methyltransferase; HEL-S-98n; catechol O-methyltransferase; epididymis secretory sperm binding protein Li 98n; testicular tissue protein Li 42; EC 2.1.1.6
• Gene ID: 1312
• mRNA Refseq: NM_000754
• Protein Refseq: NP_000745
• MIM: 116790
• UniProt ID: P21964