Recombinant Human CRK Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CRK-5279H
• Product Overview: CRK MS Standard C13 and N15-labeled recombinant protein (NP_005197) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of an adapter protein family that binds to several tyrosine-phosphorylated proteins. The product of this gene has several SH2 and SH3 domains (src-homology domains) and is involved in several signaling pathways, recruiting cytoplasmic proteins in the vicinity of tyrosine kinase through SH2-phosphotyrosine interaction. The N-terminal SH2 domain of this protein functions as a positive regulator of transformation whereas the C-terminal SH3 domain functions as a negative regulator of transformation. Two alternative transcripts encoding different isoforms with distinct biological activity have been described.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 22.9 kDa
• AA Sequence: MAGNFDSEERSSWYWGRLSRQEAVA LLQGQRHGVFLVRDSSTSPGDYVLS VSENSRVSHYIINSSGPRPPVPPSP AQPPPGVSPSRLRIGDQEFDSLPAL LEFYKIHYLDTTTLIEPVSRSRQGS GVILRQEEAEYVRALFDFNGNDEED LPFKKGDILRIRDKPEEQWWNAEDS EGKRGMIPVPYVEKYRPASASVSAL IGGRTRTRPLEQKLISEEDLAANDI LDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CRK CRK proto-oncogene, adaptor protein [ Homo sapiens (human) ]
• Official Symbol: CRK
• Synonyms: CRK; v-crk sarcoma virus CT10 oncogene homolog (avian); v crk avian sarcoma virus CT10 oncogene homolog; adapter molecule crk; proto-oncogene c-Crk; p38; CRKII; FLJ38130
• Gene ID: 1398
• mRNA Refseq: NM_005206
• Protein Refseq: NP_005197
• MIM: 164762
• UniProt ID: P46108

Reviews

04/17/2022

The expression is high and the detection is convenient.

08/22/2020

The purity and biological activity are excellent, which makes the products extremely reliable.

07/13/2020

The background in the Western blot is very clean and shows good antibody specificity.

Q&As

How is the role of CRK assessed in the disease?

The role of CRK in disease can be assessed through in-depth analysis of the patient's genomic data, combined with the results of studies in cell models and animal models. This can help doctors better understand the patient's condition and develop more effective treatment plans.

How can I detect the expression level of CRK?

The expression level of CRK can be detected by immunohistochemistry, western blotting, and real-time PCR. These techniques can detect protein or mRNA levels of CRK in tissues or cells to assess changes in its expression in disease.

Which biomolecules can be used as molecular markers for CRK?

Certain biomolecules can be used as molecular markers for CRK, such as mRNA or protein of CRK detected in biological samples such as blood and urine. These markers can be used for early diagnosis of disease, prognostic judgment, or monitoring of treatment efficacy.

What are the detection methods for CRK?

Detection methods for CRK include immunohistochemistry, western blotting, real-time PCR, etc. These methods can detect the expression level of CRK in tissues or cells to assess its status in disease.

What are the treatment strategies for CRK?

Therapeutic strategies for CRK include inhibition of its activity, regulation of its expression, and gene therapy. For example, the development of small molecule inhibitors or antibody drugs against CRK to inhibit its aberrant activity in disease, or the regulation of CRK expression through gene knockout or overexpression techniques.

What is the main role of CRK in cells?

As a signal transduction molecule, CRK plays multiple roles within the cell. It can phosphorylate a variety of substrates, affecting their activity or localization. In addition, CRK is also involved in processes such as cytoskeletal reorganization and cell motility.