Active Recombinant Human CRLF2 protein, Fc/Avi-tagged, Biotinylated

• Cat.No.: CRLF2-051H
• Product Overview: Biotinylated Recombinant Human CRLF2(Gly25 & Val30-Lys231) protein, fused to Fc/Avi tag at the C-terminus, was expressed in CHO cells .

Specification

• Description: Thymic stromal lymphopoietin receptor (TSLPR), also known cytokine receptor-like factor 2(CRLF2) and IL-XR, is a member of the type 1 cytokine receptor family. TSLPR has been identified most closely related to the common gamma chain ( gamma c) and, when complexed with interleukin 7 receptor alpha (IL-7R alpha ), forms a high affinity complex for the IL-7-like cytokine TSLP (1-4). The extracellular domain (ECD) of human TSLPR contains two fibronectin type III-like domains and a WSXWS-like motif, which is necessary for proper protein folding (1-4). The cytoplasmic domain contains a membrane-proximal box 1 motif that is important for association with JAKs (1, 2). Human TSLPR shares 34% amino acid sequence identity with mouse TSLPR. An alternatively spliced mRNA variant encoding a soluble TSLPR has also been reported in mouse (5). In the signaling pathway of TSLP-TSLPR, which is similar to that of IL-7, TSLP activates the transcription factor signal transducer and activator of transcription 3 (STAT3), inducing the expression of common genes (4). TSLPR expression is ubiquitous in the immune and hematopoietic cells but is up‑regulated in Th2-skewed cells (3, 4). Elevated expression of TSLP-TSLPR in bronchial mucosa has been associated with human asthma by acting as a susceptibility factor to generate Th2 allergic responses to antigens (4, 6). TSLP also is involved in Th2-mediated allergic skin inflammation by inducing Th2 cytokine secretion by T cells during the effector phase of allergic skin inflammation (4, 7). TSLP has been shown to induce the release of T cell-attracting chemokines from monocytes and enhance the maturation of CD11c+ dendritic cells (DC) (4). TSLP activated human DCs are also involved in the homeostatic proliferation of naïve and memory T cells in the absence of foreign antigens (4).
• Source: CHO cells
• Species: Human
• Tag: Fc/Avi
• Predicted N Terminal: Gly25 and Val30
• Form: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Bio-activity: The biotin to protein ratio is greater than 0.7 as determined by the HABA assay. Measured by its binding ability in a functional ELISA. When Recombinant Human TSLP is immobilized at 0.5 μg/mL (100 μL/well), the concentration of Biotinylated Recombinant Human TSLP Fc Chimera Avi-tag that produces 50% of the optimal binding response is 0.75-7.5 ng/mL.
• Molecular Mass: 65-80 kDa, under reducing conditions
• Protein length: Gly25 & Val30-Lys231
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Applications: Bioactivity
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 °C as supplied.1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: CRLF2 cytokine receptor-like factor 2 [ Homo sapiens ]
• Official Symbol: CRLF2
• Synonyms: CRLF2; cytokine receptor-like factor 2; CRL2; TSLPR; IL-XR; TSLP receptor; P2RY8/CRLF2 fusion; cytokine receptor-like 2; cytokine receptor CRL2 precusor; thymic stromal lymphopoietin receptor; thymic stromal lymphopoietin protein receptor; thymic stromal-derived lymphopoietin receptor; CRLF2Y
• Gene ID: 64109
• mRNA Refseq: NM_001012288
• Protein Refseq: NP_001012288
• MIM: 300357
• UniProt ID: Q9HC73