Active Recombinant Human CRLF2 protein, His-tagged, Low Endotoxin

• Cat.No.: CRLF2-77H
• Product Overview: Recombinant Human CRLF2 protein(Q9HC73-1)(25-231 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: His
• Protein Length: 25-231 aa
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human TSLP, hFc Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human TSLPR, His Tag with the EC50 of 0.44μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 25.1 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: CRLF2 cytokine receptor-like factor 2 [ Homo sapiens ]
• Official Symbol: CRLF2
• Synonyms: CRLF2; cytokine receptor-like factor 2; CRL2; TSLPR; IL-XR; TSLP receptor; P2RY8/CRLF2 fusion; cytokine receptor-like 2; cytokine receptor CRL2 precusor; thymic stromal lymphopoietin receptor; thymic stromal lymphopoietin protein receptor; thymic stromal-derived lymphopoietin receptor; CRLF2Y
• Gene ID: 64109
• mRNA Refseq: NM_001012288
• Protein Refseq: NP_001012288
• MIM: 300357
• UniProt ID: Q9HC73