Recombinant Human CROT Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: CROT-5783H
• Product Overview: CROT MS Standard C13 and N15-labeled recombinant protein (NP_066974) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the carnitine/choline acetyltransferase family. The encoded protein converts 4,8-dimethylnonanoyl-CoA to its corresponding carnitine ester. This transesterification occurs in the peroxisome and is necessary for transport of medium- and long- chain acyl-CoA molecules out of the peroxisome to the cytosol and mitochondria. The protein thus plays a role in lipid metabolism and fatty acid beta-oxidation. Alternatively spliced transcript variants have been described.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 70.2 kDa
• AA Sequence: MENQLAKSTEERTFQYQDSLPSLPV PSLEESLKKYLESVKPFANQEEYKK TEEIVQKFQSGIGEKLHQKLLERAK GKRNWLEEWWLNVAYLDVRIPSQLN VNFAGPAAHFEHYWPPKEGTQLERG SITLWHNLNYWQLLRKEKVPVHKVG NTPLDMNQFRMLFSTCKVPGITRDS IMNYFRTESEGRSPNHIVVLCRGRA FVFDVIHEGCLVTPPELLRQLTYIH KKCHSEPDGPGIAALTSEERTRWAK AREYLIGLDPENLALLEKIQSSLLV YSMEDSSPHVTPEDYSEIIAAILIG DPTVRWGDKSYNLISFSNGVFGCNC DHAPFDAMIMVNISYYVDEKIFQNE GRWKGSEKVRDIPLPEELIFIVDEK VLNDINQAKAQYLREASDLQIAAYA FTSFGKKLTKNKMLHPDTFIQLALQ LAYYRLHGHPGCCYETAMTRHFYHG RTETMRSCTVEAVRWCQSMQDPSVN LRERQQKMLQAFAKHNKMMKDCSAG KGFDRHLLGLLLIAKEEGLPVPELF TDPLFSKSGGGGNFVLSTSLVGYLR VQGVVVPMVHNGYGFFYHIRDDRFV VACSAWKSCPETDAEKLVQLTFCAF HDMIQLMNSTHLTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: CROT carnitine O-octanoyltransferase [ Homo sapiens (human) ]
• Official Symbol: CROT
• Synonyms: CROT; carnitine O-octanoyltransferase; peroxisomal carnitine O-octanoyltransferase; COT; peroxisomal carnitine acyltransferase
• Gene ID: 54677
• mRNA Refseq: NM_021151
• Protein Refseq: NP_066974
• MIM: 606090
• UniProt ID: Q9UKG9