Recombinant Human CS, His-tagged

• Cat.No.: CS-259H
• Product Overview: A DNA sequence encoding the human CS (O75390) (Met1-Gly466) was expressed with a C-terminal polyhistidine tag.

Specification

• Species: Human
• Source: Insect Cells
• Tag: His
• Protein Length: Met1-Gly466
• Form: Lyophilized from sterile PBS, pH7.4.
• Molecular Mass: The secreted recombinant human CS consists of 449 amino acids and predicts a molecular mass of 50.4 KDa. The apparent molecular mass of the protein is approximately 46 KDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 eu per μg of the protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: Hardcopy of COA with reconstitution instruction is sent along with the products.

Gene Information

• Gene Name: CS citrate synthase [ Homo sapiens ]
• Official Symbol: CS
• Synonyms: CS; citrate synthase; citrate synthase, mitochondrial
• Gene ID: 1431
• mRNA Refseq: NM_004077
• Protein Refseq: NP_004068
• MIM: 118950
• UniProt ID: O75390
• Chromosome Location: 12q13.2
• Pathway: Citrate cycle (TCA cycle), organism-specific biosystem; Citrate cycle (TCA cycle), conserved biosystem; Citrate cycle, first carbon oxidation, oxaloacetate =>2-oxoglutarate, organism-specific biosystem; Citrate cycle, first carbon oxidation, oxaloacetate => 2-oxoglutarate, conserved biosystem; Citric acid cycle (TCA cycle), organism-specific biosystem
• Function: citrate (Si)-synthase activity; citrate (Si)-synthase activity; transferase activity