Recombinant Human CTGF Protein, Full Length

• Cat.No.: CTGF-20HFL
• Product Overview: Recombinant Human CTGF Protein (Full Length) without tag was expressed in Mouse myeloma cell line.

Specification

• Species: Human
• Source: Mouse myeloma cell
• Protein Length: Full Length
• Description: The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.
• Bio-activity: Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED50 for this effect is 1.5-7.5 μg/mL.
• Molecular Mass: Predicted Molecular Mass: 35 kDa
  SDS-PAGE: 35-45 kDa, reducing conditions
• Endotoxin: <0.10 EU/μg of the protein by the LAL method.
• Purity: >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• N-terminal Sequence Analysis: No results obtained. Gln27 inferred from enzymatic pyroglutamate treatment revealing Asn28
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• References: 1. Hall-Glenn, F. and K.M. Lyons (2011) Cell. Mol. Life Sci. 68:3209.
  2. Bradham, D.M. et al. (1991) J. Cell Biol. 114:1285.
  3. Gao, R. and D.R. Brigstock (2004) J. Biol. Chem. 279:8848.

Gene Information

• Gene Name: CCN2 cellular communication network factor 2 [ Homo sapiens (human) ]
• Official Symbol: CCN2
• Synonyms: CCN2; cellular communication network factor 2; CTGF; NOV2; HCS24; IBP-8; IGFBP8; CCN family member 2; IGF-binding protein 8; connective tissue growth factor; hypertrophic chondrocyte-specific protein 24; insulin-like growth factor-binding protein 8
• Gene ID: 1490
• mRNA Refseq: NM_001901
• Protein Refseq: NP_001892
• MIM: 121009
• UniProt ID: Q5M8T4