Recombinant Rat Ccn2 Protein, Full Length

• Cat.No.: CTGF-14RFL
• Product Overview: Recombinant Rat Ccn2 Protein (Full Length) without tag was expressed in Human embryonic kidney cell.

Specification

• Species: Rat
• Source: HEK293
• Protein Length: Full length
• Description: Enables fibronectin binding activity and growth factor activity. Involved in several processes, including positive regulation of G0 to G1 transition; positive regulation of collagen biosynthetic process; and positive regulation of macromolecule metabolic process. Located in several cellular components, including cell cortex; cis-Golgi network; and perinuclear region of cytoplasm. Used to study endomyocardial fibrosis. Biomarker of several diseases, including artery disease (multiple); lung disease (multiple); membranoproliferative glomerulonephritis; primary biliary cholangitis; and restrictive cardiomyopathy (multiple). Orthologous to human CCN2 (cellular communication network factor 2).
• Bio-activity: Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. The ED50 for this effect is 1-5 μg/mL
• Molecular Mass: Predicted Molecular Mass: 35 kDa
  SDS-PAGE: 35-41 kDa, reducing conditions
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
• N-terminal Sequence Analysis: No results obtained. Gln25 inferred from enzymatic pyroglutamate treatment revealing Asp26
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
• Shipping: At ambient temperature
• Reconstitution: Reconstitute at 500 μg/mL in water.
• References: 1. Hall-Glenn, F. and K.M. Lyons (2011) Cell. Mol. Life Sci. 68:3209.
  2. Xu, J. et al. (2000) J. Cell. Biochem. 77:103.
  3. Gao, R. and D.R. Brigstock (2004) J. Biol. Chem. 279:8848.

Gene Information

• Gene Name: Ccn2 cellular communication network factor 2 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Ccn2
• Synonyms: Ccn2; cellular communication network factor 2; Ctgf; CTGRP; CCN family member 2; connective tissue growth factor; connective tissue growth-related protein
• Gene ID: 64032
• mRNA Refseq: NM_022266
• Protein Refseq: NP_071602
• UniProt ID: Q9R1E9