Recombinant Human CTLA4 protein, Fc-tagged CTLA4-2234H

Recombinant Human CTLA4 protein, Fc-tagged

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Recombinant Human CTLA4 protein, Fc-tagged

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Cat.No. : CTLA4-2234H
Product Overview : Recombinant Human CTLA4 protein (Ala37-Ser160), fused to human IgG1 Fc tag at C-terminus, was expressed in human 293 cells (HEK293).
Description : CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
Source : HEK293
Species : Human
Tag : human IgG1 Fc
Form : Lyophilized from 0.22 um filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 40.0 kDa. As a result of glycosylation, the protein migrates as 45-55 kDa under reducing (R) condition, and 90 kDa under non-reducing (NR) condition (SDS-PAGE).
Protein length : 124
Endotoxin : Less than 1.0 EU per ug by the LAL method.
Purity : >98% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 centigrade to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CTLA4-2234H :

Human CTLA-4, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 98%.

Bioactivity-ELISA of CTLA4-2234H :

Immobilized Human B7-2, His Tag at 10 μg/mL (100 μL/well) can bind Human CTLA-4, Fc Tag with a linear range of 2-39 ng/mL (QC tested).

Immobilized Human CTLA-4, Fc Tag at 10 μg/mL (100 μL/well) can bind Human B7-1, His Tag with a linear range of 0.156-1.25 μg/mL (Routinely tested).

Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag : Biotinylated Human B7-1, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.8260 μg/mL (Routinely tested).

Serial dilutions of Ipilimumab were added into Human CTLA-4, Fc Tag: Biotinylated Human B7-2, Fc,Avitag binding reactions. The half maximal inhibitory concentration (IC50) is 0.1701 μg/mL (Routinely tested).

Gene Name : CTLA4
Official Symbol : CTLA4
Synonyms : CTLA4; cytotoxic T-lymphocyte-associated protein 4; celiac disease 3 , CELIAC3; cytotoxic T-lymphocyte protein 4; CD; CD28; CD152; GSE; ICOS; CD152 isoform; celiac disease 3; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated serine esterase-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; GRD4; CTLA-4; IDDM12; CELIAC3
Gene ID : 1493
mRNA Refseq : NM_001037631
Protein Refseq : NP_001032720
MIM : 123890
UniProt ID : P16410

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