|Product Overview:||Recombinant Human CTLA4(Ala 37 - Ser 160) fused with a human IgG1 Fc tag at the C-terminus was expressed in HEK293.|
|Description:||CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.|
|Predicted N Terminal:||Ala 37|
|Form:||Lyophilized from 0.22 μm filtered solution in 35 mM Tris, 50 mM NaCl, 50 mM Glycine, pH8.0. Normally trehalose is added as protectant before lyophilization.|
|Molecular Mass:||The protein has a calculated MW of 40 kDa. As a result of glycosylation, the protein migrates as 50-55 kDa under reducing (R) condition, and 100-110 kDa under non-reducing (NR) condition (SDS-PAGE).|
|Protein length:||Ala 37 - Ser 160|
|Endotoxin:||Less than 1.0 EU per μg by the LAL method.|
|Purity:||>98% as determined by SDS-PAGE.|
|Storage:||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Gene Name:||CTLA4 cytotoxic T-lymphocyte-associated protein 4 [ Homo sapiens ]|
|Synonyms:||CTLA4; cytotoxic T-lymphocyte-associated protein 4; celiac disease 3 , CELIAC3; cytotoxic T-lymphocyte protein 4; CD; CD28; CD152; GSE; ICOS; CD152 isoform; celiac disease 3; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated serine esterase-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; GRD4; CTLA-4; IDDM12; CELIAC3;|