Active Recombinant Human CTLA4 protein, mFc-Avi-tagged, Biotinylated

• Cat.No.: CTLA4-256H
• Product Overview: Biotinylated Recombinant Human CTLA4 protein(Ala 37 - Phe 162), fused with mFc and Avi tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: mFc-Avi
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human B7-1, Fc Tag (HPLC-verified) at 5 μg/mL (100 μL/well) can bind Biotinylated Humane CTLA-4, Mouse IgG2a Fc,Avitag with a linear range of 0.3-5 ng/mL.Immobilized Human B7-2, Fc Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Humane CTLA-4, Mouse IgG2a Fc,Avitag with a linear range of 0.3-2 ng/mL.Immobilized Ipilimumab at 5 μg/mL (100 μL/well) can bind Biotinylated Humane CTLA-4, Mouse IgG2a Fc,Avitag with a linear range of 0.2-2 ng/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™).The protein has a calculated MW of 42.1 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Ala37-Phe162
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: CTLA4 cytotoxic T-lymphocyte-associated protein 4 [ Homo sapiens ]
• Official Symbol: CTLA4
• Synonyms: CTLA4; cytotoxic T-lymphocyte-associated protein 4; celiac disease 3 , CELIAC3; cytotoxic T-lymphocyte protein 4; CD; CD28; CD152; GSE; ICOS; CD152 isoform; celiac disease 3; cytotoxic T-lymphocyte antigen 4; cytotoxic T-lymphocyte-associated antigen 4; cytotoxic T-lymphocyte-associated serine esterase-4; cytotoxic T lymphocyte associated antigen 4 short spliced form; ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4; GRD4; CTLA-4; IDDM12; CELIAC3
• Gene ID: 1493
• mRNA Refseq: NM_001037631
• Protein Refseq: NP_001032720
• UniProt ID: P16410