Active Recombinant Human CTSF Protein, His-tagged

• Cat.No.: CTSF-05H
• Product Overview: Recombinant human Cathepsin F protein (20-484aa), fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Description: Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W.
• Source: Insect cell
• Species: Human
• Tag: His
• Form: Liquid
• Bio-activity: Specific activity is > 5 pmol/min/μg, and is defined as the amount of enzyme that hydrolyze 1pmole of Z-Phe-Arg-AMC to Z-Phe-Arg and AMC per minute at pH 5.0 at 37 centigrade.
• Molecular Mass: 52.5kDa (474aa)
• Protein length: 20-484aa
• AA Sequence: APAQPRAASFQAWGPPSPELLAPTR FALEMFNRGRAAGTRAVLGLVRGRV RRAGQGSLYSLEATLEEPPCNDPMV CRLPVSKKTLLCSFQVLDELGRHVL LRKDCGPVDTKVPGAGEPKSAFTQG SAMISSLSQNHPDNRNETFSSVISL LNEDPLSQDLPVKMASIFKNFVITY NRTYESKEEARWRLSVFVNNMVRAQ KIQALDRGTAQYGVTKFSDLTEEEF RTIYLNTLLRKEPGNKMKQAKSVGD LAPPEWDWRSKGAVTKVKDQGMCGS CWAFSVTGNVEGQWFLNQGTLLSLS EQELLDCDKMDKACMGGLPSNAYSA IKNLGGLETEDDYSYQGHMQSCNFS AEKAKVYINDSVELSQNEQKLAAWL AKRGPISVAINAFGMQFYRHGISRP LRPLCSPWLIDHAVLLVGYGNRSDV PFWAIKNSWGTDWGEKGYYYLHRGS GACGVNTMASSAVVD
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 90% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25 mg/mL (determined by Absorbance at 280nm)
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 40% glycerol

Gene Information

• Gene Name: CTSF cathepsin F [ Homo sapiens (human) ]
• Official Symbol: CTSF
• Synonyms: CTSF; cathepsin F; CATSF; CLN13; cathepsin F; EC 3.4.22.41
• Gene ID: 8722
• mRNA Refseq: NM_003793
• Protein Refseq: NP_003784
• MIM: 603539
• UniProt ID: Q9UBX1