Recombinant Human CTSO protein, His & T7-tagged CTSO-1103H

Recombinant Human CTSO protein, His & T7-tagged

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Recombinant Human CTSO protein, His & T7-tagged

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Cat.No. : CTSO-1103H
Product Overview : Recombinant Human CTSO aa. (Arg111~Ser317 (Accession # P43234) fused with N-terminal His & T7 tag was produced in E. coli cells.
Description : The protein encoded by the gene is a cysteine proteinase and a member of the papain superfamily. This proteolytic enzyme is involved in cellular protein degradation and turnover. The recombinant form of this enzyme was shown to degrade synthetic peptides typically used as substrates for cysteine proteinases and its proteolytic activity was abolished by an inhibitor of cyteine proteinase.
Source : E. coli
Species : Human
Tag : His & T7
Form : Freeze-dried powder
Molecular Mass : Predicted Molecular Mass: 26.4kDa.
Protein length : Arg111~Ser317 (Accession # P43234)
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : >95%
Characteristic : The isoelectric point is 5.8.
Applications : SDS-PAGE; WB; ELISA; IP.
Stability : The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Storage buffer : Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Reconstitution : Reconstitute in sterile ddH2O.
SDS-PAGE :

Gene Name : CTSO cathepsin O [ Homo sapiens (human) ]
Official Symbol : CTSO
Synonyms : CTSO; cathepsin O; CTSO1
Gene ID : 1519
mRNA Refseq : NM_001334.2
Protein Refseq : NP_001325.1
UniProt ID : P43234

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