Recombinant Human CYBB Protein (283-570 aa), His-SUMO-tagged

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Recombinant Human CYBB Protein (283-570 aa), His-SUMO-tagged

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Cat.No. : CYBB-443H
Product Overview : Recombinant Human CYBB Protein (283-570 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Research Area: Cancer. Protein Description: Partial.
Description : Critical component of the mbrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from Cytoplasmic domain NADPH across the plasma mbrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H+ currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
Source : E. coli
Species : Human
Tag : His-SUMO
Form : Tris-based buffer, 50% glycerol
Molecular Mass : 49.2 kDa
Protein length : 283-570 aa
AA Sequence : ERLVRFWRSQ QKVVITKVVT HPFKTIELQM KKKGFKMEVG QYIFVKCPKV SKLEWHPFTL TSAPEEDFFS IHIRIVGDWT EGLFNACGCD KQEFQDAWKL PKIAVDGPFG TASEDVFSYE VVMLVGAGIG VTPFASILKS VWYKYCNNAT NLKLKKIYFY WLCRDTHAFE WFADLLQLLE SQMQERNNAG FLSYNIYLTG WDESQANHFA VHHDEEKDVI TGLKQKTLYG RPNWDNEFKT IASQHPNTRI GVFLCGPEAL AETLSKQSIS NSESGPRGVH FIFNKENF
Purity : > 90% as determined by SDS-PAGE.
Notes : Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
Storage : The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
Concentration : A hardcopy of COA with concentration instruction is sent along with the products.
Figure :

(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Gene Name : CYBB cytochrome b-245, beta polypeptide [ Homo sapiens ]
Official Symbol : CYBB
Synonyms : CYBB; NOX2; CGD91-phox; NADPH oxidase 2; CGD; AMCBX2; GP91-1; GP91PHOX; p91-PHOX; GP91-PHOX;
Gene ID : 1536
mRNA Refseq : NM_000397
Protein Refseq : NP_000388
MIM : 300481
UniProt ID : P04839

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