Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein, His-Avi-tagged, Biotinylated

• Cat.No.: CD28-8665H
• Product Overview: Biotinylated Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein(P10747)(Asn19-Pro152), fused with N-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Human/Cynomolgus/Rhesus macaque
• Source: HEK293
• Tag: Avi&His
• Protein Length: Asn19-Pro152
• Tag: N-His-Avi
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Anti-CD28 Antibody, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human/Cynomolgus/Rhesus macaque CD28, His Tag with the EC50 of 24.4ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 18.2 kDa. Due to glycosylation, the protein migrates to 38-50 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
• Reconstitution: Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
• Conjugation: Biotin