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Active Recombinant Human CYP3A5 Protein

Cat.No. : CYP3A5-69H
Product Overview : Recombinant human CYP3A5 and human CYP-reductase without tag was coexpressed in Escherichia coli, supplemented with purified human cytochrome b5.
Amount: 0.5 nmol cytochrome P450
  • Specification
  • Gene Information
  • Related Products
Description : This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The encoded protein metabolizes drugs as well as the steroid hormones testosterone and progesterone. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Two pseudogenes of this gene have been identified within this cluster on chromosome 7. Expression of this gene is widely variable among populations, and a single nucleotide polymorphism that affects transcript splicing has been associated with susceptibility to hypertensions. Alternative splicing results in multiple transcript variants.
Source : E. coli
Species : Human
Bio-activity : Cytochrome c Reductase Activity: 1095 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: Testosterone 6β-hydroxylase
Linear with time up to: 7 min(Determined at 60 µM substrate, using a P450 concentration of 5 pmol/ml)
Linear with P450 up to: 25 pmol/ml(Determined at 60 µM substrate, using an incubation time of 5 min)
Vmax: 59 pmol/min/pmol CYP
Km: 50 µM
Usage : For laboratory (research) purposes only.
Storage : Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration : P450 Concentration: 1.0 nmol/ml
Cytochrome b5 Concentration: 5.0 nmol/ml
Protein Concentration: 10.0 mg/ml
Specific P450 Content: 100 pmol/mg protein
Storage Buffer : 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Gene Name : CYP3A5 cytochrome P450 family 3 subfamily A member 5 [ Homo sapiens (human) ]
Official Symbol : CYP3A5
Synonyms : CYP3A5; cytochrome P450 family 3 subfamily A member 5; CP35; PCN3; CYPIIIA5; P450PCN3; cytochrome P450 3A5; aryl hydrocarbon hydroxylase; cytochrome P450 HLp2; cytochrome P450, family 3, subfamily A, polypeptide 5; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 5; cytochrome P450-PCN3; flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenase; EC 1.14.14.1
Gene ID : 1577
mRNA Refseq : NM_000777
Protein Refseq : NP_000768
MIM : 605325
UniProt ID : P20815

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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