Recombinant Human CYP4A11 Protein CYP4A11-73H

Recombinant Human CYP4A11 Protein

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Recombinant Human CYP4A11 Protein

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Cat.No. : CYP4A11-73H
Product Overview : Recombinant human CYP4A11 and human CYP-reductase without tag was coexpressed in Escherichia coli.
Amount: 0.5 nmol cytochrome P450
Description : This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates medium-chain fatty acids such as laurate and myristate. Multiple transcript variants have been found for this gene.
Source : E. coli
Species : Human
Bio-activity : Cytochrome c Reductase Activity: 232 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM Tris-HCl buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: Lauric acid 12-hydroxylase
Linear with time up to: 10 min(Determined at 10 µM substrate, using a P450 concentration of 10 pmol/ml)
Linear with P450 up to: 16 pmol/ml(Determined at 10 µM substrate, using an incubation time of 5 min)
Vmax: 9.6 pmol/min/pmol CYP
Km: 1.0 µM
Usage : For laboratory (research) purposes only.
Storage : Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration : P450 Concentration: 1.0 nmol/ml
Protein Concentration: 10.0 mg/ml
Specific P450 Content: 100 pmol/mg protein
Storage Buffer : 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Gene Name : CYP4A11 cytochrome P450 family 4 subfamily A member 11 [ Homo sapiens (human) ]
Official Symbol : CYP4A11
Synonyms : CYP4A11; cytochrome P450 family 4 subfamily A member 11; CP4Y; CYP4A2; CYP4AII; CYPIVA11; cytochrome P450 4A11; 20-HETE synthase; 20-hydroxyeicosatetraenoic acid synthase; P450HL-omega; alkane-1 monooxygenase; cytochrome P-450HK-omega; cytochrome P450, family 4, subfamily A, polypeptide 11; cytochrome P450, subfamily IVA, polypeptide 11; cytochrome P450HL-omega; fatty acid omega-hydroxylase; lauric acid omega-hydroxylase; long-chain fatty acid omega-monooxygenase; EC 1.14.14.1; EC 1.14.14.80
Gene ID : 1579
mRNA Refseq : NM_000778
Protein Refseq : NP_000769
MIM : 601310
UniProt ID : Q02928

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