Active Recombinant Human CYP4A11 Protein

• Cat.No.: CYP4A11-84H
• Product Overview: Recombinant human CYP4A11 protein and human CYP-reductase without tag were coexpressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Description: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates medium-chain fatty acids such as laurate and myristate. Multiple transcript variants have been found for this gene.
• Bio-activity: Cytochrome c Reductase Activity: 247 nmol/min/mg protein
  P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM Tris-HCl buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
  Activity: Lauric acid 12-hydroxylase
  Linear with time up to: 20 min(Determined at 10 µM substrate, using a P450 concentration of 10 pmol/ml)
  Linear with P450 up to: 120 pmol/ml(Determined at 10 µM substrate, using an incubation time of 5 min)
  Vmax: 3.5 min-1
  Km: 2.9 µM
• Usage: For laboratory (research) purposes only
• Storage: Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
• Concentration: P450 Concentration: 4.0 nmol/ml
  Protein Concentration: 7.7 mg/ml
  Specific P450 Content: 519 pmol/mg protein
• Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Gene Information

• Gene Name: CYP4A11 cytochrome P450 family 4 subfamily A member 11 [ Homo sapiens (human) ]
• Official Symbol: CYP4A11
• Synonyms: CYP4A11; cytochrome P450 family 4 subfamily A member 11; CP4Y; CYP4A2; CYP4AII; CYPIVA11; cytochrome P450 4A11; 20-HETE synthase; 20-hydroxyeicosatetraenoic acid synthase; P450HL-omega; alkane-1 monooxygenase; cytochrome P-450HK-omega; cytochrome P450, family 4, subfamily A, polypeptide 11; cytochrome P450, subfamily IVA, polypeptide 11; cytochrome P450HL-omega; fatty acid omega-hydroxylase; lauric acid omega-hydroxylase; long-chain fatty acid omega-monooxygenase
• Gene ID: 1579
• mRNA Refseq: NM_000778
• Protein Refseq: NP_000769
• MIM: 601310
• UniProt ID: Q02928