| Cat.No. : |
CYP4F3-21H |
| Product Overview : |
Recombinant human CYP4F3B and human CYP-reductase without tag was coexpressed in Escherichia coli.
Amount: 0.5 nmol cytochrome P450 |
| Description : |
This gene, CYP4F3, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F8, is approximately 18 kb away. Several transcript variants encoding two different isoforms have been found for this gene. |
| Source : |
E. coli |
| Species : |
Human |
| Bio-activity : |
Cytochrome c Reductase Activity: 435 nmol/min/mg protein
P450 Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM potassium phosphate buffer (pH 7.4) containing 5 mM magnesium chloride and an NADPH generating system [5 mM glucose 6-phosphate, 1 mM NADP+, 1 U/ml glucose 6-phosphate dehydrogenase])
Activity: Leukotriene B4 ω-hydroxylase
Linear with time up to: 15 min(Determined at 10 µM substrate, using a P450 concentration of 8 pmol/ml)
Linear with P450 up to: 20 pmol/ml(Determined at 10 µM substrate, using an incubation time of 5 min)
Vmax: 6.1 pmol/min/pmol CYP
Km: 2.2 µM |
| Usage : |
For laboratory (research) purposes only. |
| Storage : |
Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice. |
| Concentration : |
P450 Concentration: 1.0 nmol/ml
Protein Concentration: 10.0 mg/ml
Specific P450 Content: 100 pmol/mg protein |
| Storage Buffer : |
50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.