Recombinant Human CYP7A1 protein, His-tagged

• Cat.No.: CYP7A1-2872H
• Product Overview: Recombinant Human CYP7A1 protein(188-387 aa), fused to His tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 188-387 aa
• Form: The purified protein was Lyophilized from sterile PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH7.4). 5 % trehalose and 5 % mannitol are added as protectant before lyophilization. The elution buffer contain 300mM imidazole.
• AA Sequence: FGRDLTRRDTQKAHILNNLDNFKQFDKVFPALVAGLPIHMFRTAHNAREKLAESLRHENLQKRESISELISLRMFLNDTLSTFDDLEKAKTHLVVLWASQANTIPATFWSLFQMIRNPEAMKAATEEVKRTLENAGQKVSLEGNPICLSQAELNDLPVLDSIIKESLRLSSASLNIRTAKEDFTLHLEDGSYNIRKDDII
• Purity: 95%, by SDS-PAGE with Coomassie Brilliant Blue staining.
• Storage: Short-term storage: Store at 2-8°C for (1-2 weeks).
  Long-term storage: Aliquot and store at -20°C to -80°C for up to 3 months, buffer containing 50% glycerol is recommended for reconstitution. Avoid repeat freeze-thaw cycles.
• Reconstitution: Reconstitute at 0.25 µg/μl in 200 μl sterile water for short-term storage.
  Reconstitution with 200 μl 50% glycerol solution is recommended for longer term storage.

Gene Information

• Gene Name: CYP7A1 cytochrome P450, family 7, subfamily A, polypeptide 1 [ Homo sapiens ]
• Official Symbol: CYP7A1
• Synonyms: CYP7A1; cytochrome P450, family 7, subfamily A, polypeptide 1; CYP7, cytochrome P450, subfamily VIIA (cholesterol 7 alpha monooxygenase), polypeptide 1; cholesterol 7-alpha-monooxygenase; cholesterol 7 alpha monooxygenase; cytochrome P450 7A1; cholesterol 7-alpha-hydroxylase; cytochrome P450, subfamily VIIA polypeptide 1; CP7A; CYP7; CYPVII; MGC126826; MGC138389
• Gene ID: 1581
• mRNA Refseq: NM_000780
• Protein Refseq: NP_000771
• MIM: 118455
• UniProt ID: P22680