Recombinant Human cytomegalovirus (HCMV) gB protein(Met1-Lys700 & Arg777-Val907), Fc-tagged

• Cat.No.: gB-1045C
• Product Overview: Recombinant Human CMV gB (AAA45920.1, with furin cleavage site mutated from 'RTKR' to 'TTQT') (Arg 777-Val 907)(Met 1-Lys 700)precursor was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: CMV
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Lys700 & Arg777-Val907
• Form: Lyophilized from sterile 100mM Gly, 10mM NaCl, 50mM Tris, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to bind biotinylated Human CD209-Fc in functional ELISA.
• Molecular Mass: The recombinant human CMV glycoprotein B/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 1045 amino acids and has a predicted molecular mass of 118 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of gB/Fc monomer is approximately 160-170 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.