Active Recombinant Human cytomegalovirus (CMV) gB protein, Fc-tagged

• Cat.No.: gB-1045C
• Product Overview: Recombinant Human cytomegalovirus (CMV) gB protein(Met 1-Lys 700&Arg 777-Val 907)(AAA45920.1,with furin cleavage site mutated from 'RTKR' to 'TTQT') precursor was expressed in HEK293, fused with the Fc region of human IgG1 at the C-terminus.

Specification

• Species: CMV
• Source: HEK293
• Tag: Fc
• Protein Length: 1-700&777-907 aa
• Form: Lyophilized from sterile 100mM Gly, 10mM NaCl, 50mM Tris, pH 7.5.
• Bio-activity: Measured by its ability to bind biotinylated Human CD209-Fc in functional ELISA.
• Molecular Mass: The recombinant human CMV glycoprotein B/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 1045 amino acids and has a predicted molecular mass of 118 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of gB/Fc monomer is approximately 160-170 kDa due to glycosylation.
• Endotoxin: < 1 EU/µg as determined by LAL test.
• Purity: > 80 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Official Symbol: gB