Recombinant Human DAP3 protein(49-398 aa), GST-tagged

• Cat.No.: DAP3-11824H
• Product Overview: Recombinant Human DAP3 protein(49-398 aa)(P51398), fused with N-terminal GST tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: GST
• Protein Length: 49-398 aa
• Form: Lyophilized from sterile PBS, pH 8.5, 5% trehalose, 5% mannitol.
• Molecular Mass: The protein has a calculated MW of 66 kDa.
• AASequence: MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLVPRGSSRTNENDPAKHGDQHEGQHYNISPQDLETVFPHGLPPRFVMQVKTFSEACLMVRKPALELLHYLKNTSFAYPAIRYLLYGEKGTGKTLSLCHVIHFCAKQDWLILHIPDAHLWVKNCRDLLQSSYNKQRFDQPLEASTWLKNFKTTNERFLNQIKVQEKYVWNKRESTEKGSPLGEVVEQGITRVRNATDAVGIVLKELKRQSSLGMFHLLVAVDGINALWGRTTLKREDKSPIAPEELALVHNLRKMMKNDWHGGAIVSALSQTGSLFKPRKAYLPQELLGKEGFDALDPFIPILVSNYNPKEFESCIQYYLENNWLQHEKAPTEEGKKELLFLSNANPSLLERHCAYL
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: > 85 % as determined by SDS-PAGE.
• Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.165 mg/ml. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: DAP3 death associated protein 3 [ Homo sapiens ]
• Official Symbol: DAP3
• Synonyms: DAP3; death associated protein 3; 28S ribosomal protein S29, mitochondrial; bMRP 10; DAP 3; DKFZp686G12159; MGC126058; MGC126059; mitochondrial 28S ribosomal protein S29; MRP S29; MRPS29; S29mt; death-associated protein 3; ionizing radiation resistance conferring protein; DAP-3; MRP-S29; bMRP-10; FLJ12817
• Gene ID: 7818
• mRNA Refseq: NM_001199849
• Protein Refseq: NP_001186778
• MIM: 602074
• UniProt ID: P51398

Case Study

Case 1: Han J, et al. Sci Adv. 2020

DAP3, identified as an oncogenic RNA editing suppressor, binds ADAR2's deaminase domain to block its interaction with target RNAs, exemplified by PDZD7's stop codon recoding (Stop→Trp). Tumor cells accumulate unedited PDZD7WT—more tumorigenic than edited PDZD7Stop518W—due to DAP3-mediated editing repression. This mechanism highlights how cancer exploits RNA editome dysregulation via secondary regulators like DAP3 to enhance malignancy and survival.

Fig1. Co-IP analysis of the nuclear and cytoplasmic fractions of EC109 cells transfected with DAP3-GFP or EV-GFP.

Fig2. WB analysis of DAP3 protein in xenograft tumors at end point.

Case 2: Jia Y, et al. Br J Cancer. 2014

DAP3, a proapoptotic member of the DAP family, serves as a prognostic marker in gastric cancer, with higher tumor expression correlating to improved survival and preoperative chemotherapy response. Clinical analysis of 87 patients and in vitro studies revealed that DAP3 knockdown promotes tumor migration, chemoresistance, and reduced apoptosis via suppressed caspase-3 activation, emphasizing its dual role in apoptosis regulation and therapeutic outcome prediction. These findings highlight DAP3's potential as a biomarker for optimizing combined therapy efficacy and prognosis assessment in gastric cancer.

Fig1. DAP3 expression knock down at mRNA and protein level in AGS and HGC27 cell lines.

Fig2. Flow cytometric analysis of apoptosis of AGS PEF and DAP3 cell lines under normal conditions.

Application

1. Therapeutic Potential of Recombinant DAP3 Protein in Cancer Management Recombinant DAP3 protein, a proapoptotic regulator, shows promise in enhancing chemotherapy efficacy by restoring apoptosis in cancer cells. Studies reveal its role in counteracting chemoresistance, particularly in gastric cancer, where low endogenous DAP3 correlates with poor treatment response. By modulating caspase-3 activation, recombinant DAP3 may sensitize tumors to chemotherapeutic agents, offering a novel adjuvant therapy to improve clinical outcomes. 2. Diagnostic and Prognostic Applications As a biomarker, recombinant DAP3 aids in evaluating preoperative chemotherapy effectiveness and predicting prognosis. High DAP3 expression in tumors is linked to improved survival rates, enabling personalized treatment strategies. Its integration into diagnostic assays could refine risk stratification and guide therapeutic decisions, aligning with precision oncology frameworks. 3. Research and Drug Development Implications In vitro, recombinant DAP3 suppresses cancer cell migration and invasion, highlighting its dual role in apoptosis induction and metastasis inhibition. These properties position it as a candidate for targeted drug development. Ongoing research explores its synergy with immunotherapy and small-molecule inhibitors, potentially unlocking combinatorial approaches to combat chemoresistance and tumor progression.

Fig1. The signaling pathway and function of DAP3. (Hao Song, 2024)