Recombinant Human DDOST Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: DDOST-6049H
• Product Overview: DDOST MS Standard C13 and N15-labeled recombinant protein (NP_005207) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 50.7 kDa
• AA Sequence: MGYFRCAGAGSFGRRRKMEPSTAAR AWALFWLLLPLLGAVCASGPRTLVL LDNLNVRETHSLFFRSLKDRGFELT FKTADDPSLSLIKYGEFLYDNLIIF SPSVEDFGGNINVETISAFIDGGGS VLVAASSDIGDPLRELGSECGIEFD EEKTAVIDHHNYDISDLGQHTLIVA DTENLLKAPTIVGKSSLNPILFRGV GMVADPDNPLVLDILTGSSTSYSFF PDKPITQYPHAVGKNTLLIAGLQAR NNARVIFSGSLDFFSDSFFNSAVQK AAPGSQRYSQTGNYELAVALSRWVF KEEGVLRVGPVSHHRVGETAPPNAY TVTDLVEYSIVIQQLSNGKWVPFDG DDIQLEFVRIDPFVRTFLKKKGGKY SVQFKLPDVYGVFQFKVDYNRLGYT HLYSSTQVSVRPLQHTQYERFIPSA YPYYASAFSMMLGLFIFSIVFLHMK EKEKSDTRTRPLEQKLISEEDLAAN DILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: DDOST dolichyl-diphosphooligosaccharide--protein glycosyltransferase [ Homo sapiens (human) ]
• Official Symbol: DDOST
• Synonyms: DDOST; dolichyl-diphosphooligosaccharide--protein glycosyltransferase; dolichyl diphosphooligosaccharide protein glycosyltransferase; dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit; KIAA0115; OST; OST48; WBP1; DDOST 48 kDa subunit; advanced glycation endproduct receptor 1; oligosaccharyltransferase 48 kDa subunit; oligosaccharyl transferase 48 kDa subunit; dolichyl-diphosphooligosaccharide-protein glycotransferase; dolichyl-diphosphooligosaccharide-protein glycosyltransferase; AGER1; CDG1R; OKSWcl45; MGC2191
• Gene ID: 1650
• mRNA Refseq: NM_005216
• Protein Refseq: NP_005207
• MIM: 602202
• UniProt ID: P39656