Recombinant Human DESI1, His-tagged

• Cat.No.: DESI1-15886H
• Product Overview: Recombinant human DESI1 protein, fused to His tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-168aa
• Description: DESI1 belongs to the DeSI family and contains 1 PPPDE peptidase domain. This protein is a protease which deconjugates SUMO1, SUMO2 and SUMO3 from some substrate proteins and has isopeptidase but not SUMO-processing activity. DESI1 desumoylates ZBTB46.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
• Molecular Mass: 20.7 kDa (191aa), confirmed by MALDI-TOF
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEPPNLY PVKLYVYDLS KGLARRLSPI MLGKQLEGIW HTSIVVHKDE FFFGSGGISS CPPGGTLLGP PDSVVDVGST EVTEEIFLEY LSSLGESLFR GEAYNLFEHN CNTFSNEVAQ FLTGRKIPSY ITDLPSEVLS TPFGQALRPL LDSIQIQPPG GSSVGRPNGQ S
• Purity: >85% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term. For long term storage, aliquot and store at at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.25mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: DESI1 desumoylating isopeptidase 1 [ Homo sapiens (human) ]
• Official Symbol: DESI1
• Synonyms: DESI1; desumoylating isopeptidase 1; DESI2; DeSI-1; PPPDE2; FAM152B; D15Wsu75e; DJ347H13.4; desumoylating isopeptidase 2; PPPDE peptidase domain containing 2; PPPDE peptidase domain-containing protein 2; family with sequence similarity 152, member B
• Gene ID: 27351
• mRNA Refseq: NM_015704
• Protein Refseq: NP_056519
• MIM: 614637
• UniProt ID: Q6ICB0
• Chromosome Location: 22q13.2