Recombinant Human DGKB, His-tagged

• Cat.No.: DGKB-11954H
• Product Overview: Recombinant Human DGKB protein, fused to His-tag, was expressed in E.coli and purified by Ni-sepharose.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 30-140a.a.
• Description: Diacylglycerol kinases (DGKs) are regulators of the intracellular concentration of the second messenger diacylglycerol (DAG) and thus play a key role in cellular processes. Nine mammalian isotypes have been identified, which are encoded by separate genes. Mammalian DGK isozymes contain a conserved catalytic (kinase) domain and a cysteine-rich domain (CRD). The protein encoded by this gene is a diacylglycerol kinase, beta isotype. Two alternatively spliced transcript variants have been found for this gene.
• Storage: The protein is stored in PBS buffer at -20℃. Avoid repeated freezing and thawing cycles.
• Storage Buffer: 1M PBS (58mM Na2HPO4,17mM NaH2PO4, 68mM NaCl, pH8. ) added with 300mM Imidazole and 0.7% Sarcosyl, 15%glycerol.

Gene Information

• Gene Name: DGKB diacylglycerol kinase, beta 90kDa [ Homo sapiens ]
• Official Symbol: DGKB
• Synonyms: DGKB; diacylglycerol kinase, beta 90kDa; DAGK2, diacylglycerol kinase, beta (90kD); diacylglycerol kinase beta; DGK; DGK BETA; KIAA0718; DAG kinase beta; diglyceride kinase beta; 90 kDa diacylglycerol kinase; DAGK2; DGK-BETA
• Gene ID: 1607
• mRNA Refseq: NM_004080
• Protein Refseq: NP_004071
• MIM: 604070
• UniProt ID: Q9Y6T7
• Chromosome Location: 7p21.2
• Pathway: Effects of PIP2 hydrolysis, organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; Glycerolipid metabolism, organism-specific biosystem; Glycerolipid metabolism, conserved biosystem; Glycerophospholipid metabolism, organism-specific biosystem; Glycerophospholipid metabolism, conserved biosystem
• Function: ATP binding; calcium ion binding; diacylglycerol kinase activity; nucleotide binding; transferase activity